Molecular Biology Problem Solver 40 pps

... Problems are inevitable, so taking a systematic approach to troubleshooting will, in the long run, save time, Western Blotting 389 Western Blotting 391 Protein remains in the gel Were there problems ... problems with contact or arrangement of the blotting apparatus? Were there problems with reagents? Has the protein high molecular weight? Was transfer time sufficient? Are the buffer componen...

Ngày tải lên: 02/07/2014, 04:21

... 156–159 of reagents, 39, 40 41, 41–42 of restriction endonucleases, 232 of Western blots, 383 of Western blotting antibodies, 384 Storage conditions, as source of problems, 21–22, 40 42 Storage phosphor ... purification, 210 Resolution of autoradiography film, 438 improving in electrophoresis, 353 of labels, 405 406 of pH meters, 85 of spectrophotometers, 97–98 of storage phosphor imagers,...

Ngày tải lên: 02/07/2014, 04:21

... Equipment 87 change due to temperature as they would in a manual calibration. A second source of problems is not matching a buffer or group of buffers with the correct buffer set in the meter.

Ngày tải lên: 02/07/2014, 04:21

... causing the problem. Is the Sample the Problem? If the sample reading seems inappropriate, measure the pH of a buffer standard. A correct measurement of the standard points to a sample problem. ... recom- mended. Sometimes the “expected” value is not obtained for a mea- surement but the correct value is. The problem is simply an incorrect perceived value. Competing ions, sodium ions...

Ngày tải lên: 02/07/2014, 04:21

... Radioactivity on the Outside of the Vial. Does This Indicate a Problem? . . . . . . . . . . . . 151 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright ... enterococci and staphylococci on hospital fabrics and plastic. J. Clin. Microbiol. 38:724–726. 140 Haidaris and Brownlow do the work will benefit you in the long run. A courteous, consid- er...

Ngày tải lên: 02/07/2014, 04:21

... fully below. A Wipe Test Detected Radioactivity on the Outside of the Vial. Does This Indicate a Problem? If you detect contamination on the outside of your vial, contact your RSO. She will tell

Ngày tải lên: 02/07/2014, 04:21

... DNA and subsequent ethanol washes eliminate such a contaminant. Phenol can also be problematic. If you experience problems with DNA purified by a phenol-based strategy, apply chloroform to extract ... based on yields pub- lished for samples similar if not identical to your own. Do You Require High Molecular Weight Material? The average size of genomic DNA prepared will vary between commercia...

Ngày tải lên: 02/07/2014, 04:21

... R., Kingston, R. E., Moore, D. D., Seidman, J. G., and Struhl, K. 1998. Current Protocols in Molecular Biology. Wiley, New York. Azad, A. K., Coote, J. G., and Parton, R. 1992. An improved method ... from solutions are between 50 to 95% and from agarose gels, 40 to 80%. Fragments smaller than 100bp or larger than 10kb (gel), or 50 kb (solution), are problematic. Small fragments may not e...

Ngày tải lên: 02/07/2014, 04:21

... total RNA isolation. In this section we address problems presented by difficult tissues and offer troubleshooting techniques to help over- come these problems. A separate section will discuss the ... isolating total RNA from plant tissue using a non-phenol- based method, polyvinylpyrrolidone -40 (PVP -40) can be added to the lysate to absorb polysaccharide and polyphenolic contami- nants. Whe...

Ngày tải lên: 02/07/2014, 04:21

... 60–150 polymerase (40 60 at 95°C. depending on protein concentration a,b Stoffel fragment Absent Absent 21 at 97.5°C 5–10 130 Tth DNA Absent Present 25 polymerase rTth XL Trace Present 30 40 AmpliTaq ... 50 at 97.5°C polymerase (low) Pfu DNA Present Absent 1 140 at 95°C a 10 a 60 polymerase (native and recombinant) Pfu DNA Absent Absent 1 140 at 95°C a 11 a polymerase (exo-form) (Pyrococc...

Ngày tải lên: 02/07/2014, 04:21