Molecular Biology Problem Solver 39 pps

... considering protein blot quantitation. Standards (known amounts of purified target protein—not to be confused with molecular weight stan- dards) must be run on every blot, since even with the most con- sistent technique ... accurate records. Consistency and good record-keeping will make it much easier to isolate the source of any problem that may come up later. Which Transfer Membrane Is M...

Ngày tải lên: 02/07/2014, 04:21

... for eukaryotic expression, 504–506 Stains high background, 362, 395 396 for nucleic acids (table), 360 for nucleic acid transfer, 419–420 problems with, 395 for proteins (table), 360 selecting for electrophoresis, 357–363 Standard ... 350–353 native PAGE versus, 345–348 problems with, 480–481 standardized gels for, 363 Sealed containment rooms, in biosafety, 117 Secondary antibodies, 384...

Ngày tải lên: 02/07/2014, 04:21

... Equipment 87 change due to temperature as they would in a manual calibration. A second source of problems is not matching a buffer or group of buffers with the correct buffer set in the meter.

Ngày tải lên: 02/07/2014, 04:21

... causing the problem. Is the Sample the Problem? If the sample reading seems inappropriate, measure the pH of a buffer standard. A correct measurement of the standard points to a sample problem. ... recom- mended. Sometimes the “expected” value is not obtained for a mea- surement but the correct value is. The problem is simply an incorrect perceived value. Competing ions, sodium ions...

Ngày tải lên: 02/07/2014, 04:21

... Radioactivity on the Outside of the Vial. Does This Indicate a Problem? . . . . . . . . . . . . 151 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright ... decontaminated. Consult your institutional guidelines on Working Safely with Biological Samples 139 Requesting the Media Room to Sterilize Labware Identify Your Goods Use black permanent ink...

Ngày tải lên: 02/07/2014, 04:21

... fully below. A Wipe Test Detected Radioactivity on the Outside of the Vial. Does This Indicate a Problem? If you detect contamination on the outside of your vial, contact your RSO. She will tell

Ngày tải lên: 02/07/2014, 04:21

... DNA and subsequent ethanol washes eliminate such a contaminant. Phenol can also be problematic. If you experience problems with DNA purified by a phenol-based strategy, apply chloroform to extract ... based on yields pub- lished for samples similar if not identical to your own. Do You Require High Molecular Weight Material? The average size of genomic DNA prepared will vary between commercia...

Ngày tải lên: 02/07/2014, 04:21

... R., Kingston, R. E., Moore, D. D., Seidman, J. G., and Struhl, K. 1998. Current Protocols in Molecular Biology. Wiley, New York. Azad, A. K., Coote, J. G., and Parton, R. 1992. An improved method ... method for the prepa- ration of highly purified plasmid DNA from Escherichia coli. Anal. Biochem. 212 :394 –401. Fernley, H. N. 1971. In Boyer, P. D., ed., The Enzymes, vol. 4. Chapter 2, Mamma...

Ngày tải lên: 02/07/2014, 04:21

... total RNA isolation. In this section we address problems presented by difficult tissues and offer troubleshooting techniques to help over- come these problems. A separate section will discuss the ... RNA- binding filters. Increasing the ratio of sample mass: volume of lysis buffer can help alleviate this problem in filter-based isolations. Multiple acid–phenol extractions can be done to ensure...

Ngày tải lên: 02/07/2014, 04:21

... electrophoresis may not allow you to reach the conclusion that there are two different mRNA forms if their molecular weights are similar.You might require a separation technique with greater resolving power. Suppose ... primer degradation by the 3¢–5¢ exonuclease activity (de Noronha and Mullins, 1992 and Skerra, 1992).The problem of reduced yield can be corrected by including an enzyme w...

Ngày tải lên: 02/07/2014, 04:21