Molecular Biology Problem Solver 30 docx

... . . . . . . . . . . . . . . . . . . . . . . . 309 How Can Sample Preparation Affect Your Results? . . . . . . . 311 291 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. ... appropriate PCR strategy, to avoid problems, and to help you think your way out of problems that do arise. While your research topic may be unique, the solutions to most PCR problems are les...

Ngày tải lên: 02/07/2014, 04:21

... the sample is not allowed to reach the temperature of the weighing instrument. This is especially problematic when weighing very small samples. You the researcher are also a source of moisture that ... moisture (up to 400 micrograms [mg]). The body will also transfer heat to the sample, creating problems addressed above. Sample Location The sample should be centered as much as possible on the...

Ngày tải lên: 02/07/2014, 04:21

... Dispense the test volume into the weighing vessel as follows: • Place the filled tip at an angle of 30 against the inside of the weighing vessel • Dispense the test volume slowly and uniformly up ... movement develops at the junction contributing a small elec- Table 4.4 Pipette Troubleshooting Guide Problem Possible Cause Solution Pipette drips or Tip is loose or does Use manufacturer recom...

Ngày tải lên: 02/07/2014, 04:21

... E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds., Current Protocols in Molecular Biology. Wiley, New York, pp. 10.1.1–10.1.3. US Pharmacopeia, USP645. Voet, D., and Voet, J. ... for them to check with their physician as to the potential hazards to them of working in a microbiology lab where even organisms that are normally nonpathogenic are being handled. The Inoculati...

Ngày tải lên: 02/07/2014, 04:21

... no. G304), 0.5 mg DNA in 50 ml NEB Buffer 2 for 1 hour at 37°C. AvrII added at 20 units and diluted to 10 units, etc., with reaction mix. The marker NEB Low Range PFG Marker (cat. no. N 0305 0S). Complete ... enzymes are active, and the restriction sites are within several bases of each other, there may be a problem cutting close to the end of the fragment. Sequential Enzyme sets that are...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 387 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... markers will provide precise molecular weight values. The pre-stained recombinant proteins of known, reproducible molecular weights discussed above can also determine the molecular we...

Ngày tải lên: 02/07/2014, 04:21

... obtain a known gene of interest include purchase from a distributor of clones from the Integrated Molecular Analysis of Genomes and their Expression (IMAGE) consortium (http://image.llnl.gov/), ... inability to obtain homogeneously pure protein for crys- tallization is a frequently encountered problem due to the het- erogeneous carbohydrate content of many eukaryotic proteins (Gruening...

Ngày tải lên: 02/07/2014, 04:21

... transient assays (e.g., in COS cells). Once you have ruled out problems with the expression con- struct, there are a few obvious places where problems may be occurring. First, one could perform Northern ... sequences. In some cases an overlooked point mutation or mistake in the original design is the problem. Ideally such prob- lems are best uncovered before weeks of work have been devoted t...

Ngày tải lên: 02/07/2014, 04:21

... 86 during pipette testing, 73, 75 in plasmid purification, 180–181 for polymerase chain reactions, 302 303 , 309 –310 of restriction enzyme shipments, 259–260 storage phosphor imagers and, 446 in storing ... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442–443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems with,...

Ngày tải lên: 02/07/2014, 04:21