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Molecular Biology Problem Solver 22 ppsx
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RNA isolation. In this section we address problems presented by
difficult tissues and offer troubleshooting techniques to help over-
come these problems. A separate section will discuss the ... RNA-
binding filters. Increasing the ratio of sample mass: volume of lysis
buffer can help alleviate this problem in filter-based isolations.
Multiple acid–phenol extractions can be done to ensure...
Molecular Biology Problem Solver 58 ppsx
... 234–235
star activity of, 229 –230
Restriction enzymes, 226 –262
altering specificity of, 248–250
commercially available, 226 227
complex digests with, 239–244
costs of, 227 228
double digests with, ... hybridization target, 402
solutions of polymers of, 287–288
storage of, 214–215, 219, 222
RNA purification, 198 222
integrity of RNA from, 202–203
lysis in, 215
maximizing yield from, 2...
Molecular Biology Problem Solver 15 ppsx
... Radioactivity on the Outside
of the Vial. Does This Indicate a Problem? . . . . . . . . . . . . 151
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright ... particulate
matter by centrifugation and a prefiltration step can help prevent
clogging of the 0.45 or 0 .22 micron filters used to remove bacte-
ria and fungi. These filters will not remove my...
Molecular Biology Problem Solver 16 ppsx
... fully below.
A Wipe Test Detected Radioactivity on the Outside of the
Vial. Does This Indicate a Problem?
If you detect contamination on the outside of your vial, contact
your RSO. She will tell
Molecular Biology Problem Solver 18 ppsx
... DNA and subsequent ethanol washes eliminate such a
contaminant. Phenol can also be problematic. If you experience
problems with DNA purified by a phenol-based strategy, apply
chloroform to extract ... based on yields pub-
lished for samples similar if not identical to your own.
Do You Require High Molecular Weight Material?
The average size of genomic DNA prepared will vary between
commercia...
Molecular Biology Problem Solver 31 ppsx
... electrophoresis may not allow you to reach the conclusion that
there are two different mRNA forms if their molecular weights
are similar.You might require a separation technique with greater
resolving power.
Suppose ... primer
degradation by the 3¢–5¢ exonuclease activity (de Noronha and
Mullins, 1992 and Skerra, 1992).The problem of reduced yield can
be corrected by including an enzyme w...
Molecular Biology Problem Solver 50 ppsx
... . . . . . . . 521
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 2001 by Wiley-Liss, Inc.
ISBNs: 0-471-37972-7 (Paper); 0-471 -223 90-5 (Electronic)
transmembrane, ... Production Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . 531
Mutation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....
Molecular Biology Problem Solver 54 ppsx
... Acad.
Sci. U.S.A. 95:2509–2514.
Higgins, D. R., and Cregg, J. M. 1998. Pichia Protocols: Methods in Molecular
Biology, vol 103. Humana Press, Totowa NJ.
Hill-Perkins, M. S., and Possee, R. D. 1990. A baculovirus ... use of smaller transfer vectors (<6kb)
often eliminates such genetic instabilities.
Solubility Problems
In general, many recombinant proteins that are insoluble in E.
coli b...
Molecular Biology Problem Solver 57 ppsx
... 411
Probe templates, in hybridization
experiments, 402
Problem reduction, 2
Problem resolution, failed, 28
Problems. See also Global problems;
Technical problems
with acrylamide polymerization,
342–343
Index ... preparation for, 311–312
troubleshooting, 315– 322
Web sites for, 328–329
PCR strategies
developing, 293–296, 296–315
in RNA purification, 200
troubleshooting, 221 222
weak link...
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