Molecular Biology Problem Solver 21 doc

... Preparation . . . 212 What Constitutes “RNase-Free Technique”? . . . . . . . . . . . . 212 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... Reused? . . . 211 Can a Kit Designed to Isolate mRNA Directly from the Biological Sample Purify mRNA from Total RNA? . . . 212 Maximizing the Yield and Quality of an RNA Pr...

Ngày tải lên: 02/07/2014, 04:21

... the sample is not allowed to reach the temperature of the weighing instrument. This is especially problematic when weighing very small samples. You the researcher are also a source of moisture that ... moisture (up to 400 micrograms [mg]). The body will also transfer heat to the sample, creating problems addressed above. Sample Location The sample should be centered as much as possible on the...

Ngày tải lên: 02/07/2014, 04:21

... button and pull the tip up along the inside of the weighing vessel. Release the control button. 4. Document the value that appears in the display of the balance immediately after the display has ... movement develops at the junction contributing a small elec- Table 4.4 Pipette Troubleshooting Guide Problem Possible Cause Solution Pipette drips or Tip is loose or does Use manufacturer recomm...

Ngày tải lên: 02/07/2014, 04:21

... E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds., Current Protocols in Molecular Biology. Wiley, New York, pp. 10.1.1–10.1.3. US Pharmacopeia, USP645. Voet, D., and Voet, J. ... for them to check with their physician as to the potential hazards to them of working in a microbiology lab where even organisms that are normally nonpathogenic are being handled. The Inoculati...

Ngày tải lên: 02/07/2014, 04:21

... Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G. and Struhl, K. 1995. Current Protocols in Molecular Biology. Wiley, New York. Bashyam, M., and Tyage, A. 1994. An efficient and high yielding method ... method for iso- lation of RNA from Mycobacteria. Biotech. 17:834–836. Brown, T. A., ed. 1991. Molecular Biology Labfax. Bios Scientific Publishers, Oxford, U.K. Cheung, A. L., Eberhar...

Ngày tải lên: 02/07/2014, 04:21

... . . . 241 Double Digests: Simultaneous or Sequential? . . . . . . . . . . . . 242 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–169. Croy, R. D., ed. 1993. Plant Molecular Biology. Bios Scientific Publishers, Oxford, U.K. Fang, G., Hammar, S., and Gr...

Ngày tải lên: 02/07/2014, 04:21

... enzymes are active, and the restriction sites are within several bases of each other, there may be a problem cutting close to the end of the fragment. Sequential Enzyme sets that are not compatible ... megabase mapping can be found in Nelson and McClelland (1992) and Qiang et al. (1990). A potential problem is that certain methyla- tion sites may react slowly allowing partial cleavage eve...

Ngày tải lên: 02/07/2014, 04:21

... . . . . 309 How Can Sample Preparation Affect Your Results? . . . . . . . 311 291 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... appropriate PCR strategy, to avoid problems, and to help you think your way out of problems that do arise. While your research topic may be unique, the solutions to most PCR problems are le...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 387 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... markers will provide precise molecular weight values. The pre-stained recombinant proteins of known, reproducible molecular weights discussed above can also determine the molecular we...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 475 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... Res. 22:3418–3422. Zhu, Z., and Waggoner, A. S. 1997. Molecular mechanism controlling the incor- poration of fluorescent nucleotides into DNA by PCR. Cytometry 28:206 211 . 461 15 E. coli...

Ngày tải lên: 02/07/2014, 04:21