Molecular Biology Problem Solver 19 pptx

... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442–443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems with, 447–448 quantitative ... 350–353 native PAGE versus, 345–348 problems with, 480–481 standardized gels for, 363 Sealed containment rooms, in biosafety, 117 Secondary antibodies, 384 problems with, 395 Secondar...

Ngày tải lên: 02/07/2014, 04:21

... MOLECULAR BIOLOGY PROBLEM SOLVER Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue, New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail: PERMREQ@WILEY.COM. This p...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic) nique ... Project Will Define You 2 Which Research Style Best Fits Your Situation? . . . . . . . . . . 2 Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2 Expe...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . 19 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic) Big ... . . . . . . . . . . . . . 13 Does a Company Test Every Application for a Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 How Well Will...

Ngày tải lên: 02/07/2014, 04:21

... the wrong temperature. Solving Problems by Yourself In a perfect world, six steps will solve any problem: 1. Define the problem. What do you see? The first step in any problem- solving activity is ... nature of the problem without drawing conclusions. Understanding a problem consists of describing all the factual aspects about the problem. Do not try to determine what caused the probl...

Ngày tải lên: 02/07/2014, 04:21

... estimation above. The rationale behind these two steps is that a properly chosen buffer will have a 50 :50 ratio of acid to base at the target pH, therefore you will have 10¥ the available capacity ... . . . . . . . . . . 44 What Organics Can Be Present in the Water? . . . . . . . . . . . 45 What Other Problems Occur in Water Systems?. . . . . . . . . 46 Bibliography . . . . . . . . . . . ....

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . . . 67 Data on the performance characteristics of different protein concentration assays were generously provided by Bio Rad Inc. Molecular Biology Problem Solver: A Laboratory ... . . . . . . . . . . . . 67 What Are the Elements of Proper Pipetting Technique? . . . 68 Preventing and Solving Problems . . . . . . . . . . . . . . . . . . . . . . . 68 Troubleshoot...

Ngày tải lên: 02/07/2014, 04:21

... 4.2 Centrifuge Application Guide Low Speed High Speed Ultra Application (70 00 rpm/ (30,000 rpm/ (100,000 rpm/ for Pelleting 70 00 ¥ g) 100,000 ¥ g) 1,000,000 ¥ g) Cells Yes Yes Feasible, but not recommended Nuclei ... the Visible Range? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 07 What Are the Strategies to Determine the Extinction Coefficient of a Compoun...

Ngày tải lên: 02/07/2014, 04:21

... Properly Use and Maintain Laboratory Equipment 61 68 Troutman et al. adjustable pipette, set at 100 ml has an inaccuracy specification of ±0 .8% , whereas a Series 2100 100 ml fixed-volume pipettes ... move freely on the piston. Overall pipette design is similar from one brand to the next (Figure 4 .8) , but each pipette has a few slightly different features. Always refer to the manufacturer’...

Ngày tải lên: 02/07/2014, 04:21

... a problem. Limitations If phenolic contaminants (i.e., from plants) are a problem, adding 1% polyvinylpyrrolidine to your extraction buffer can absorb them (John, 199 2; Pich and Schubert, 199 3; ... al., 199 8). Recombination impaired hosts are often selected when producing plasmids prone to deletion and rearrangement of cloned inserts (Summers and Sherratt, 198 4; Biek and Cohen,...

Ngày tải lên: 02/07/2014, 04:21

... desirable. Inhibition may become a problem if the DNA has contaminants that influence enzyme activity. Salt and other contaminants in the DNA solution are more likely to be problematic if the DNA solution ... of PmeI will digest 1mg of DNA in two hours at 37°C but can digest 2 mg lambda in two hours at 25 C (Robinson, D., unpublished observation). When using PmeI for digesting agarose–embed...

Ngày tải lên: 02/07/2014, 04:21

... water is also recommended, especially if one is proceeding to another step where EDTA might be a problem. The Volume of Enzyme in the Vial Appears Very Low. Did Leakage Occur during Shipment? Some ... nucleases. TROUBLESHOOTING What Can Cause a Simple Restriction Digestion to Fail? Faulty Enzyme or Problem Template Preparation? If the suspect enzyme fails to digest a second or control ta...

Ngày tải lên: 02/07/2014, 04:21

... Department of Microbiology, University of Cape Town. In Molecular Biology Techniques Manual: Third Molecular Biology Techniques Manual, V. E. Coyne et al., eds. http://www.uct.ac.za/microbiology/pcroptim.htm Standard ... http://www.biotechlab.nwu.edu/pe/index.html Molecular Current Protocols in Molecular Biology biology methods http://www.wiley.com/cp/cpmb/mb0317.htm Elsevier T...

Ngày tải lên: 02/07/2014, 04:21

... methylene blue staining upon subsequent hybridization. Some newer dyes (SYBR ® Gold and SYBR ® Green, Molecular Probes Inc.) are promoted as noninterfering stains. Otherwise, in light of the inconsistencies ... times. Linearity of transfer needs to be tested empirically with dilution series of nucleic acid molecular weight markers. What Are the Indicators of a Functional Membrane? Membran...

Ngày tải lên: 02/07/2014, 04:21