Molecular Biology Problem Solver 15 ppsx

... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442–443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems with, 447–448 quantitative ... 350–353 native PAGE versus, 345–348 problems with, 480–481 standardized gels for, 363 Sealed containment rooms, in biosafety, 117 Secondary antibodies, 384 problems with, 395 Secondar...

Ngày tải lên: 02/07/2014, 04:21

... MOLECULAR BIOLOGY PROBLEM SOLVER Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue, New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail: PERMREQ@WILEY.COM. This p...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic) nique ... Project Will Define You 2 Which Research Style Best Fits Your Situation? . . . . . . . . . . 2 Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2 Expe...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . 19 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic) Big ... . . . . . . . . . . . . . 13 Does a Company Test Every Application for a Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 How Well Will...

Ngày tải lên: 02/07/2014, 04:21

... the wrong temperature. Solving Problems by Yourself In a perfect world, six steps will solve any problem: 1. Define the problem. What do you see? The first step in any problem- solving activity is ... nature of the problem without drawing conclusions. Understanding a problem consists of describing all the factual aspects about the problem. Do not try to determine what caused the probl...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . . . 150 A Wipe Test Detected Radioactivity on the Outside of the Vial. Does This Indicate a Problem? . . . . . . . . . . . . 151 Molecular Biology Problem Solver: A Laboratory ... Requirements of Autoclaving? The minimum requirements for sterilization are 15 lb of pres- sure per square inch at 115 C for 15 minutes of sterilization time (not including the exhaus...

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... . . . . . . . 164 How Can You Concentrate a Radioactive Solution? . . . . . . 164 Bibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166 Appendix ... . . . . . . . . . . . . 161 What Can You Do to Achieve Minimum Radioactive Dose? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162 How Can You Organ...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . 185 Silica Resin-Based Strategies . . . . . . . . . . . . . . . . . . . . . . . . . . . 186 Isolation from Electrophoresis Gels . . . . . . . . . . . . . . . . . . . . 187 What Are ... DNA and subsequent ethanol washes eliminate such a contaminant. Phenol can also be problematic. If you experience problems with DNA purified by a phenol-based strategy, apply chloroform to extr...

Ngày tải lên: 02/07/2014, 04:21

... total RNA isolation. In this section we address problems presented by difficult tissues and offer troubleshooting techniques to help over- come these problems. A separate section will discuss the ... RNA- binding filters. Increasing the ratio of sample mass: volume of lysis buffer can help alleviate this problem in filter-based isolations. Multiple acid–phenol extractions can be done to ensure...

Ngày tải lên: 02/07/2014, 04:21

... electrophoresis may not allow you to reach the conclusion that there are two different mRNA forms if their molecular weights are similar.You might require a separation technique with greater resolving power. Suppose ... primer degradation by the 3¢–5¢ exonuclease activity (de Noronha and Mullins, 1992 and Skerra, 1992).The problem of reduced yield can be corrected by including an enzyme w...

Ngày tải lên: 02/07/2014, 04:21

... 521 Planning the Baculovirus Experiment . . . . . . . . . . . . . . . . . . . . . 521 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... an Appropriate Expression Host . . . . . . . . . . . . . . . 501 Selecting an Appropriate Expression Vector . . . . . . . . . . . . . 506 Implementing the Eukaryotic Expression Expe...

Ngày tải lên: 02/07/2014, 04:21

... Acad. Sci. U.S.A. 95:2509–2514. Higgins, D. R., and Cregg, J. M. 1998. Pichia Protocols: Methods in Molecular Biology, vol 103. Humana Press, Totowa NJ. Hill-Perkins, M. S., and Possee, R. D. 1990. A baculovirus ... baculovirus-infected insect cells: Expression, purification and characterization. Prot. Expr. Purif. 10: 154 161. Gignac, S. M., Brauer, S., Hane, B., Quentmeier, H., and Drexle...

Ngày tải lên: 02/07/2014, 04:21

... hybridization experiments, 402 Problem reduction, 2 Problem resolution, failed, 28 Problems. See also Global problems; Technical problems with acrylamide polymerization, 342–343 Index 557 Ink, for marking ... (piperazine diacrylamide), as crosslinker, 338 Peer-reviewed journals, 5 Pelleting, 56, 57, 65 improving, 66–67 Pellets, problems with RNA, 220–221 Peptide electrophoresis, buffer...

Ngày tải lên: 02/07/2014, 04:21