Molecular Biology Problem Solver 9 docx
Molecular Biology Problem Solver 58 ppsx
... conditions, as source of
problems, 21–22, 40–42
Storage phosphor imagers
dynamic range of, 442–443
erasure of, 447
in nucleic acid hybridization,
441–448
operation of, 441
problems with, 447–448
quantitative ... 350–353
native PAGE versus, 345–348
problems with, 480–481
standardized gels for, 363
Sealed containment rooms, in
biosafety, 117
Secondary antibodies, 384
problems with, 395
Secondar...
Molecular Biology Problem Solver 1 pdf
... MOLECULAR BIOLOGY
PROBLEM SOLVER
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 20 01 by Wiley-Liss, Inc.
ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue,
New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail:
PERMREQ@WILEY.COM.
This p...
Molecular Biology Problem Solver 2 potx
... . . . . . . . . 9
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 20 01 by Wiley-Liss, Inc.
ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic)
nique ... Project Will Define
You 2
Which Research Style Best Fits Your Situation? . . . . . . . . . . 2
Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2
Expe...
Molecular Biology Problem Solver 3 pdf
... . . . . . . . 19
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 2001 by Wiley-Liss, Inc.
ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic)
Big ... . . . . . . . . . . . . . 13
Does a Company Test Every Application for a
Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
How Well Will...
Molecular Biology Problem Solver 4 pptx
... the wrong temperature.
Solving Problems by Yourself
In a perfect world, six steps will solve any problem:
1. Define the problem. What do you see?
The first step in any problem- solving activity is ... nature of the problem without drawing conclusions.
Understanding a problem consists of describing all the factual
aspects about the problem. Do not try to determine what caused
the probl...
Molecular Biology Problem Solver 5 ppt
... estimation above.
The rationale behind these two steps is that a properly chosen
buffer will have a 50 :50 ratio of acid to base at the target pH,
therefore you will have 10¥ the available capacity ... . . . . . . . . . . 44
What Organics Can Be Present in the Water? . . . . . . . . . . . 45
What Other Problems Occur in Water Systems?. . . . . . . . . 46
Bibliography . . . . . . . . . . . ....
Molecular Biology Problem Solver 7 docx
... 4.2 Centrifuge Application Guide
Low Speed High Speed Ultra
Application (70 00 rpm/ (30,000 rpm/ (100,000 rpm/
for Pelleting 70 00 ¥ g) 100,000 ¥ g) 1,000,000 ¥ g)
Cells Yes Yes Feasible, but not
recommended
Nuclei ... the
Visible Range? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 07
What Are the Strategies to Determine the Extinction
Coefficient of a Compoun...
Molecular Biology Problem Solver 9 docx
... How to Properly Use and Maintain Laboratory Equipment 79
trical voltage to the overall measurement system. Generally this
is a minor error. If the flow ... The fill solution most
often contains potassium chloride, but incompatibility with some
Figure 4 .9 Double-junction
combination electrode. Rep-
roduced with permission from
Thermo Orion Inc.
How ... liquid surface of the weighing vessel sh...
Molecular Biology Problem Solver 13 docx
... E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds., Current
Protocols in Molecular Biology. Wiley, New York, pp. 10.1.1–10.1.3.
US Pharmacopeia, USP645.
Voet, D., and Voet, J. ... for them to check with their physician as to
the potential hazards to them of working in a microbiology lab
where even organisms that are normally nonpathogenic are being
handled.
The Inoculati...
Molecular Biology Problem Solver 26 docx
... enzymes are active, and the restriction sites are
within several bases of each other, there may be a problem cutting
close to the end of the fragment.
Sequential
Enzyme sets that are not compatible ... megabase
mapping can be found in Nelson and McClelland (1992) and
Qiang et al. (1990). A potential problem is that certain methyla-
tion sites may react slowly allowing partial cleavage eve...
Molecular Biology Problem Solver 30 docx
... . . . . . . . . . . . . . . . . . . . . . . . 309
How Can Sample Preparation Affect Your Results? . . . . . . . 311
291
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. ... appropriate
PCR strategy, to avoid problems, and to help you think your way
out of problems that do arise. While your research topic may be
unique, the solutions to most PCR problems are les...
Molecular Biology Problem Solver 38 docx
... . . . . . . . . . . . . . . . 385
Amplification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 387
Molecular Biology Problem Solver: A Laboratory Guide. Edited ... markers will provide precise molecular weight values.
The pre-stained recombinant proteins of known, reproducible
molecular weights discussed above can also determine the
molecular...
Molecular Biology Problem Solver 51 docx
... obtain a known gene of
interest include purchase from a distributor of clones from
the Integrated Molecular Analysis of Genomes and their
Expression (IMAGE) consortium (http://image.llnl.gov/), ... inability to obtain homogeneously pure protein for crys-
tallization is a frequently encountered problem due to the het-
erogeneous carbohydrate content of many eukaryotic proteins
(Gruening...
Molecular Biology Problem Solver 53 docx
... transient assays
(e.g., in COS cells).
Once you have ruled out problems with the expression con-
struct, there are a few obvious places where problems may be
occurring. First, one could perform Northern ... sequences. In some cases an overlooked point mutation or
mistake in the original design is the problem. Ideally such prob-
lems are best uncovered before weeks of work have been devoted
t...
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