Molecular Biology Problem Solver 8 potx

... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442–443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems with, 447–448 quantitative ... 350–353 native PAGE versus, 345–348 problems with, 480–481 standardized gels for, 363 Sealed containment rooms, in biosafety, 117 Secondary antibodies, 384 problems with, 395 Secondar...

Ngày tải lên: 02/07/2014, 04:21

... MOLECULAR BIOLOGY PROBLEM SOLVER Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue, New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail: PERMREQ@WILEY.COM. This p...

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... . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic) nique ... Project Will Define You 2 Which Research Style Best Fits Your Situation? . . . . . . . . . . 2 Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2 Expe...

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... . . . . . . . 19 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic) Big ... . . . . . . . . . . . . . 13 Does a Company Test Every Application for a Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 How Well Will...

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... the wrong temperature. Solving Problems by Yourself In a perfect world, six steps will solve any problem: 1. Define the problem. What do you see? The first step in any problem- solving activity is ... nature of the problem without drawing conclusions. Understanding a problem consists of describing all the factual aspects about the problem. Do not try to determine what caused the probl...

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... estimation above. The rationale behind these two steps is that a properly chosen buffer will have a 50 :50 ratio of acid to base at the target pH, therefore you will have 10¥ the available capacity ... . . . . . . . . . . 44 What Organics Can Be Present in the Water? . . . . . . . . . . . 45 What Other Problems Occur in Water Systems?. . . . . . . . . 46 Bibliography . . . . . . . . . . . ....

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... . . . . . . . . . . 67 Data on the performance characteristics of different protein concentration assays were generously provided by Bio Rad Inc. Molecular Biology Problem Solver: A Laboratory ... . . . . . . . . . . . . 67 What Are the Elements of Proper Pipetting Technique? . . . 68 Preventing and Solving Problems . . . . . . . . . . . . . . . . . . . . . . . 68 Troubleshoot...

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... 4.2 Centrifuge Application Guide Low Speed High Speed Ultra Application (70 00 rpm/ (30,000 rpm/ (100,000 rpm/ for Pelleting 70 00 ¥ g) 100,000 ¥ g) 1,000,000 ¥ g) Cells Yes Yes Feasible, but not recommended Nuclei ... the Visible Range? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 07 What Are the Strategies to Determine the Extinction Coefficient of a Compoun...

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... Properly Use and Maintain Laboratory Equipment 61 68 Troutman et al. adjustable pipette, set at 100 ml has an inaccuracy specification of ±0 .8% , whereas a Series 2100 100 ml fixed-volume pipettes ... move freely on the piston. Overall pipette design is similar from one brand to the next (Figure 4 .8) , but each pipette has a few slightly different features. Always refer to the manufacturer’...

Ngày tải lên: 02/07/2014, 04:21

... How to Properly Use and Maintain Laboratory Equipment 79 trical voltage to the overall measurement system. Generally this is a minor error. If the flow ... The fill solution most often contains potassium chloride, but incompatibility with some Figure 4 .9 Double-junction combination electrode. Rep- roduced with permission from Thermo Orion Inc. How ... liquid surface of the weighing vessel sh...

Ngày tải lên: 02/07/2014, 04:21

... Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (eds.), 1998. Current Protocols in Molecular Biology. Wiley, New York. Beckman-Coulter Corporation. Application Note A—1790A. 1995. Biochrom ... 1988). 108 Troutman et al. Smith (1987) lists compounds that interfere with each assay and illustrates problems associated with the use of BSA as a standard (see also Harlow and Lane, 198...

Ngày tải lên: 02/07/2014, 04:21

... environmental contamination persists, work in a laminar flow biosafety hood. If a contamination problem persists in the lab, consider an examination of the lab’s air delivery system. Placing filters ... it’s a trade-off between that and soaking the plate with the condensation. In the clinical microbiology lab, blood agar plates that contain cultures under evaluation are stored at the benchtop...

Ngày tải lên: 02/07/2014, 04:21

... principle here is that impurities in the water cause problems, and the purest water avail- able should be used for electrophoresis to help prevent these problems. Bacteria in your water purifier can also ... not controlled, the pore size will vary from day to day. Symptoms of Problems with Catalyst Potency The best indicator of a problem catalyst is poor polymeriza- tion of the gel. If you’...

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... can be used to report only approximate molecular weights (within 10,000 daltons of the molecular weight as determined by an unstained standard). The molecular weight values of most pre-stained ... to Determine the Molecular Weight of an Unknown Protein? Pre-stained protein standards usually run as broad, fuzzy bands, making them useful for approximate, but not exact, molecular we...

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