Molecular Biology Problem Solver 7 docx

... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442–443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems with, 447–448 quantitative ... 350–353 native PAGE versus, 345–348 problems with, 480–481 standardized gels for, 363 Sealed containment rooms, in biosafety, 117 Secondary antibodies, 384 problems with, 395 Secondar...

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... MOLECULAR BIOLOGY PROBLEM SOLVER Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue, New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail: PERMREQ@WILEY.COM. This p...

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... . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic) nique ... Project Will Define You 2 Which Research Style Best Fits Your Situation? . . . . . . . . . . 2 Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2 Expe...

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... . . . . . . . 19 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic) Big ... . . . . . . . . . . . . . 13 Does a Company Test Every Application for a Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 How Well Will...

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... the wrong temperature. Solving Problems by Yourself In a perfect world, six steps will solve any problem: 1. Define the problem. What do you see? The first step in any problem- solving activity is ... nature of the problem without drawing conclusions. Understanding a problem consists of describing all the factual aspects about the problem. Do not try to determine what caused the probl...

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... estimation above. The rationale behind these two steps is that a properly chosen buffer will have a 50 :50 ratio of acid to base at the target pH, therefore you will have 10¥ the available capacity ... . . . . . . . . . . 44 What Organics Can Be Present in the Water? . . . . . . . . . . . 45 What Other Problems Occur in Water Systems?. . . . . . . . . 46 Bibliography . . . . . . . . . . . ....

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... 4.2 Centrifuge Application Guide Low Speed High Speed Ultra Application (70 00 rpm/ (30,000 rpm/ (100,000 rpm/ for Pelleting 70 00 ¥ g) 100,000 ¥ g) 1,000,000 ¥ g) Cells Yes Yes Feasible, but not recommended Nuclei ... the Visible Range? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 07 What Are the Strategies to Determine the Extinction Coefficient of a Compoun...

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... How to Properly Use and Maintain Laboratory Equipment 79 trical voltage to the overall measurement system. Generally this is a minor error. If the flow ... The fill solution most often contains potassium chloride, but incompatibility with some Figure 4 .9 Double-junction combination electrode. Rep- roduced with permission from Thermo Orion Inc. How ... liquid surface of the weighing vessel sh...

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... E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds., Current Protocols in Molecular Biology. Wiley, New York, pp. 10.1.1–10.1.3. US Pharmacopeia, USP645. Voet, D., and Voet, J. ... for them to check with their physician as to the potential hazards to them of working in a microbiology lab where even organisms that are normally nonpathogenic are being handled. The Inoculati...

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... enzymes are active, and the restriction sites are within several bases of each other, there may be a problem cutting close to the end of the fragment. Sequential Enzyme sets that are not compatible ... megabase mapping can be found in Nelson and McClelland (1992) and Qiang et al. (1990). A potential problem is that certain methyla- tion sites may react slowly allowing partial cleavage eve...

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... . . . . . . . . . . . . . . . . . . . . . . . 309 How Can Sample Preparation Affect Your Results? . . . . . . . 311 291 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. ... appropriate PCR strategy, to avoid problems, and to help you think your way out of problems that do arise. While your research topic may be unique, the solutions to most PCR problems are les...

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... . . . . . . . . . . . . . . . 385 Amplification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 387 Molecular Biology Problem Solver: A Laboratory Guide. Edited ... markers will provide precise molecular weight values. The pre-stained recombinant proteins of known, reproducible molecular weights discussed above can also determine the molecular...

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... obtain a known gene of interest include purchase from a distributor of clones from the Integrated Molecular Analysis of Genomes and their Expression (IMAGE) consortium (http://image.llnl.gov/), ... inability to obtain homogeneously pure protein for crys- tallization is a frequently encountered problem due to the het- erogeneous carbohydrate content of many eukaryotic proteins (Gruening...

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... transient assays (e.g., in COS cells). Once you have ruled out problems with the expression con- struct, there are a few obvious places where problems may be occurring. First, one could perform Northern ... sequences. In some cases an overlooked point mutation or mistake in the original design is the problem. Ideally such prob- lems are best uncovered before weeks of work have been devoted t...

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