Molecular Biology Problem Solver 5 ppt

... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442–443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems with, 447–448 quantitative ... 350–353 native PAGE versus, 345–348 problems with, 480–481 standardized gels for, 363 Sealed containment rooms, in biosafety, 117 Secondary antibodies, 384 problems with, 395 Secondar...

Ngày tải lên: 02/07/2014, 04:21

... MOLECULAR BIOLOGY PROBLEM SOLVER Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue, New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail: PERMREQ@WILEY.COM. This p...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic) nique ... Project Will Define You 2 Which Research Style Best Fits Your Situation? . . . . . . . . . . 2 Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2 Expe...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . 19 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic) Big ... . . . . . . . . . . . . . 13 Does a Company Test Every Application for a Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 How Well Will...

Ngày tải lên: 02/07/2014, 04:21

... the wrong temperature. Solving Problems by Yourself In a perfect world, six steps will solve any problem: 1. Define the problem. What do you see? The first step in any problem- solving activity is ... nature of the problem without drawing conclusions. Understanding a problem consists of describing all the factual aspects about the problem. Do not try to determine what caused the probl...

Ngày tải lên: 02/07/2014, 04:21

... estimation above. The rationale behind these two steps is that a properly chosen buffer will have a 50 :50 ratio of acid to base at the target pH, therefore you will have 10¥ the available capacity ... . . . . . . . . . . 44 What Organics Can Be Present in the Water? . . . . . . . . . . . 45 What Other Problems Occur in Water Systems?. . . . . . . . . 46 Bibliography . . . . . . . . . . . ....

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . . . 67 Data on the performance characteristics of different protein concentration assays were generously provided by Bio Rad Inc. Molecular Biology Problem Solver: A Laboratory ... . . . . . . . . . . . . 67 What Are the Elements of Proper Pipetting Technique? . . . 68 Preventing and Solving Problems . . . . . . . . . . . . . . . . . . . . . . . 68 Troubleshoot...

Ngày tải lên: 02/07/2014, 04:21

... a problem. Limitations If phenolic contaminants (i.e., from plants) are a problem, adding 1% polyvinylpyrrolidine to your extraction buffer can absorb them (John, 199 2; Pich and Schubert, 199 3; ... al., 199 8). Recombination impaired hosts are often selected when producing plasmids prone to deletion and rearrangement of cloned inserts (Summers and Sherratt, 198 4; Biek and Cohen,...

Ngày tải lên: 02/07/2014, 04:21

... desirable. Inhibition may become a problem if the DNA has contaminants that influence enzyme activity. Salt and other contaminants in the DNA solution are more likely to be problematic if the DNA solution ... of PmeI will digest 1mg of DNA in two hours at 37°C but can digest 2 mg lambda in two hours at 25 C (Robinson, D., unpublished observation). When using PmeI for digesting agarose–embed...

Ngày tải lên: 02/07/2014, 04:21

... water is also recommended, especially if one is proceeding to another step where EDTA might be a problem. The Volume of Enzyme in the Vial Appears Very Low. Did Leakage Occur during Shipment? Some ... nucleases. TROUBLESHOOTING What Can Cause a Simple Restriction Digestion to Fail? Faulty Enzyme or Problem Template Preparation? If the suspect enzyme fails to digest a second or control ta...

Ngày tải lên: 02/07/2014, 04:21

... from from 25 to 37 mg per A 260 unit. This approach is inexact, but it is reliable for common molecular biology techniques as long as its limitations are considered. Computer software that predicts ... and counter-ions (lithium or sodium, depending on the manufacturer), which signficantly contribute to the total molecular weight of the nucleotide preparation. Unless you consider the salt...

Ngày tải lên: 02/07/2014, 04:21

... Department of Microbiology, University of Cape Town. In Molecular Biology Techniques Manual: Third Molecular Biology Techniques Manual, V. E. Coyne et al., eds. http://www.uct.ac.za/microbiology/pcroptim.htm Standard ... http://www.biotechlab.nwu.edu/pe/index.html Molecular Current Protocols in Molecular Biology biology methods http://www.wiley.com/cp/cpmb/mb0317.htm Elsevier T...

Ngày tải lên: 02/07/2014, 04:21

... methylene blue staining upon subsequent hybridization. Some newer dyes (SYBR ® Gold and SYBR ® Green, Molecular Probes Inc.) are promoted as noninterfering stains. Otherwise, in light of the inconsistencies ... times. Linearity of transfer needs to be tested empirically with dilution series of nucleic acid molecular weight markers. What Are the Indicators of a Functional Membrane? Membran...

Ngày tải lên: 02/07/2014, 04:21

... ac- celerants. Rate accelerators can increase the hybridization rate several-fold, but if background is problematic, reduce the concentration to 5%. The performance of dextran sulfate (and perhaps other ... in boxes is higher than in bags or bottles, but these larger volumes can help reduce background problems. Larger capacity also makes it feasible to simultaneously manipulate multiple filt...

Ngày tải lên: 02/07/2014, 04:21