... where the side-chains of the hydrophobic part of the helix play a key role in receptor binding and activa-tion [54], and the 20–41 region, where side-chains have aprominent role in structural conservation ... rather helical structure (84% of residues), which isconsidered to be the active conformation of the hormone[38,40]. The changes engineered in the sequence of the peptide, either through the ... the nature of the side chain of the new amino acids or through their non-native conforma-tional features, increase the peptide–CRH complex stabilitywith the following consequences: they (a) differentiate...