PROTEIN INTERACTIONS potx

... for elucidating protein protein interactions and selecting specific binders to novel target proteins, and the first and second minireviews focus on the detection of protein protein interactions [8,9]. ... [58,59]. The generation of recombinant binding proteins from small scaffold proteins will also help to explain the mecha- nism of protein protein interactions. Consequently, analysi...

Ngày tải lên: 06/03/2014, 11:20

... our assay to detect BRET caused by protein protein interactions, light emission spectra were recorded from Cos7 cells transfected with pRluc-N2 or the two fusion proteins pRluc/GFP 2 (pBRET+) and ... oligomerization of GPCRs and other protein protein interactions [34–40]. In these studies, BRET has been measured using Rluc and enhanced yellow fluorescent protein (EYFP) as luminescent...

Ngày tải lên: 08/03/2014, 09:20

... Transient RNA protein interactions in RNA folding FEBS Journal 278 (2011) 1634–1642 ª 2011 The Authors Journal compilation ª 2011 FEBS 1641 REVIEW ARTICLE Transient RNA protein interactions in ... summarize the properties of the three protein classes, with the main focus being on RNA chaperones and annealer proteins. Specifically binding proteins A specific protein cofactor binds to i...

Ngày tải lên: 14/02/2014, 19:20

... Montoya Transient protein DNA ⁄ RNA interactions FEBS Journal 278 (2011) 1643–1650 ª 2011 The Authors Journal compilation ª 2011 FEBS 1645 REVIEW ARTICLE Transient DNA ⁄ RNA -protein interactions Francisco ... full-length protein can be extremely rewarding, as shown by the information obtained with the tumor suppressor protein BRCA2 and its interac- tion with DNA [44,45]. As compare...

Ngày tải lên: 14/02/2014, 19:20

... not observed when MSR was replaced by nNOSred or CPR, highlighting the need for specific protein protein interactions between MSR and hMS. Reactivation of hMS was found to be dependent on NADPH ... Nat Struct Biol 9, 53–56. 34 Tollin G (1995) Use of flavin photochemistry to probe intraprotein and interprotein electron transfer mecha- nisms. J Bioenerg Biomembr 27, 303–309. Supporting inform...

Ngày tải lên: 18/02/2014, 08:20

... a protein. In Protein Structure (Creighton, T.E., ed.), 2nd edn, pp. 253–259. Oxford University Press, Oxford. 35. Privalov, P.L. (1979) Stability of proteins: small globular proteins. Adv. Protein ... (enzyme I of the PTS), the first protein in the cascade of proteins forming the PTS, to HPr, the histidine-phosphocarrier protein. HPr is the smallest protein in the protein cascade...

Ngày tải lên: 19/02/2014, 12:20

... identification of protein interactions. In these techniques, protein protein Abbreviations EGFR, epidermal growth factor receptor; EGFP, enhanced green fluorescent protein; GFP, green fluorescent protein; ... detection of protein protein interactions In general, highly sensitive systems might detect even undesirable, feeble signals. To confirm the specificity of detection of protein pr...

Ngày tải lên: 05/03/2014, 23:20

... – protein protein interactions Yeast–mammal chimeric Ga system Medici et al. [78] constructed an intelligent system for analysis of protein protein interactions by managing heterotrimeric G -protein ... signaling-based screening systems for protein protein interactions Small monomeric G -protein signaling in yeast Small monomeric G-proteins, such as Ras and Ras-like proteins, ar...

Ngày tải lên: 06/03/2014, 11:20

... substrates. Protein protein interactions Multisite phosphorylation per se is not associated with switch-like responses [18,48]. We showed here that the characteristics of protein protein interactions, ... that determine the protein protein interactions: the concen- trations of the enzymes relative to the target protein, and the respective dissociation constants. Figure 5A shows th...

Ngày tải lên: 07/03/2014, 10:20

... producing the protein of interest as a cleavable fusion with a highly expressing protein. Low solubility can result from failure of the protein to fold properly, aggregation of folded protein, or ... code for ‘unique proteins’ that yield soluble pro- tein is only about one-third for prokaryotic proteins and much lower for eukaryotic proteins. In this con- text, a unique protein is defi...

Ngày tải lên: 07/03/2014, 12:20