Báo cáo khoa học " In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes " pdf

... Israilides et al. / Phytomedicine 15 (2008) 512–519516 Phytomedicine 15 (2008) 512–519 In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes C. Israilides a,Ã , ... controls. DNA synthesis assay In this assay, the rate of novel DNA synthesis in the cell nuclei is monitored, based on the incorporation of radiolabell...

Ngày tải lên: 28/06/2014, 11:20

... fusion protein immediately after the arginine of the new epitope, and removed the fragment of the PRAI (ba) 8 -barrel corresponding to a 6 (ba) 7)8 . The remaining fragment of PRAI consisted of 130 ... importantly, the residues contributing to one of the hydrogen bonds in the core of the barrel, Lys258 (b1) and Gln332 (b4), remain in trPRAI. It is temptin...

Ngày tải lên: 30/03/2014, 20:20

... mutagenesis of the active-site cysteine residue(s) to selectively eliminate catalysis in each of the individual domains and in all possible combinations of domains. Inactivation of this cysteine has ... and a three- domain adenylate kinase [24]. The present study provides insight into the inter-dependent functional properties of the three domains of FlgCK, and...

Ngày tải lên: 16/03/2014, 06:20

... But we avoided the interference of such functionalizing agent in determining the bactericidal activity of the antibiotics. Using the com- bined reducing property of antibiotics and borohydride, antibiotics ... breakage in the b-lactam ring of the free ampicillin, whereas Gnps conjugation might stabilize the ring and thereby allowing the delocalization of elec...

Ngày tải lên: 22/06/2014, 06:20

... to their growth. Further, in one of the control experiments we determined the zone of inhibition with the mixture of previously synthesized Gnps and antibiotics. In that case, the zone of inhibition did ... But we avoided the interference of such functionalizing agent in determining the bactericidal activity of the antibiotics. Using the com- bined reducing...

Ngày tải lên: 22/06/2014, 19:20

... different orientation of the substrate w ithin the substrate binding site of PAT2, and this may simultaneously avoid the interference of the polar side ch ain w ith c orresponding amino acid residues in the binding ... spectrum that includes not only t he amino acids glycine, alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D -e...

Ngày tải lên: 07/03/2014, 16:20

... the expression of ferrochelatase, which plays a role in the iron-removal reaction of exogenous heme and the change in position of the heme-moiety of hemopro- teins. The protoporphyrin ring of the heme-moiety ... (E) Ferrochelatase activity in outer and inner membranes. Zinc-insertion and iron-removal activities were measured in the outer and inner membr...

Ngày tải lên: 16/03/2014, 00:20

... Ca 2+ -calmodulin, changing the shape of the regulatory enzyme, thus impeding its inter- action at the calmodulin binding site on nNOS [17]. There are other examples of small helical peptides inhibiting nNOS ... oxygenase domain that binds heme, tetrahydrobiopterin and the substrate arginine, and (b) the electron supplying reductase domain that binds NADPH, FAD and FMN. Co...

Ngày tải lên: 17/03/2014, 09:20

... that even in the synthesis of nondisulfide bridge-containing glycopep- tides the yield is dramatically affected by the scale of the reaction, the excess of amino acids used, and the level of purity ... of the sequence. Three-fold excess of amino acid derivatives were used in the c oup ling reactions with the exception of per- Scheme 1. Sequence of additi...

Ngày tải lên: 23/03/2014, 13:20

... footprint. Letters to the right of each panel indicate the DNA sequence (5¢ end in the upper part) of the corresponding strand in this region. In the lower part, the sequence of the binding site ... position of the footprint. Letters to the right of each panel indicate the DNA sequence (5¢ end in the upper part) of the corresponding strand in this...

Ngày tải lên: 23/03/2014, 13:20