... kB, h and R as Boltzmann’s constant, Planck’sconstant and gas constant, respectively, and T and DG#as absolute temperature and Gibbs free energy of activation, respectively, the DG# of unfolding ... of unfolding and autoproteolysis of G8C ⁄ N60C and pWT. In contrast to previous studieson the kinetics of thermoinactivation [13,24], thisapproach allows quantification of the contribution of Fig. ... constants of unfolding were found todecrease in an exponential manner with increasingamounts of isopropanol. At 20% (v ⁄ v) isopropanol,no further decrease of the observed rate constants of unfolding...