Báo cáo khoa học " Characterization of stipe and cap powders of mushroom (Lentinus edodes) prepared by different grinding methods " ppt

... six different powders were obtained as: shear pulverized cap (SPC) and stipe (SPS) pow- ders; mechanically milled cap (MMC) and stipe (MMS) powders and jet milled cap (JMC) and stipe (JMS) powders. ... protein contents of cap powders (16.71–18.29 g/100 g) were much higher than that of stipe powders (5.61–7.20 g/ Table 2 Effect of grinding methods o...

Ngày tải lên: 28/06/2014, 11:20

... 164° and )169° in the A-chain and B- chain of nPS, respectively. The v 1 torsion angle for Asp35 changes from ) 75.20° to )171.65° and )89.25° in the A-chain and B-chain of nPS, and in the case of His37, ... imidazole rings of His34 in the A-chain and B-chain of nPS move by  4A ˚ and 5.5 A ˚ , whereas the backbone Ca atom moves by only  0.9 A ˚ and  0.5 A ˚ ,...

Ngày tải lên: 16/02/2014, 09:20

... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and ... 7.0 and 4 °C for indicated periods of time. Lane c, the nicked precursor was incubated at pH 7.0 and 4 °C for 24 h and then dialyzed overnight at 4 °C and pH 4.0. (B) Effec...

Ngày tải lên: 19/02/2014, 07:20

... splicing. A variety of tools are available to us for this purpose and further investigation of these sequence variants will not only further our understanding of the splicing mechanism and improve clinical ... (mouse–rat and human–dog) [10]. This analysis showed a strong puri- fying selection at silent sites in a critical region of this gene [10], spanning the 3¢ end of exon 1...

Ngày tải lên: 06/03/2014, 09:22

... BC, DE and FG loops Y/A/S/D library in all the CDR loops [ref 38] Y/S library in all the CDRs of heavy chain and CDR3 of light chain [ref 39] 10 FN3 Randomization in CDR loops of heavy and light ... peptide sequences and small scaffold proteins, and as methods for designing high-quality libraries of small scaffold proteins. Functionalization of small scaffold proteins b...

Ngày tải lên: 06/03/2014, 11:20

... analysis of the total cross section of mat foundation (b) Stress dispersion of the total cross section of mat foundation during and after curing (c) TCI of the total cross section of mat ... history can be changed by the temperature of concrete placement, the size of member, and delayed time. Table 1. Comparison between measured and analyzed hydration heat valu...

Ngày tải lên: 07/03/2014, 03:20

... by determining the apparent K m values of glycine and a lanine at pH values of pH 5.5–8.5 (Fig. 4) and a broad range of membrane potentials. At hyperpolarized membrane potentials K m values of ... also osmolytes such as sarcosine and betaine, and the D -enantiomers of serine and alanine. The apparent affinities of PAT1 substrates are mainly in the range of 2–15 m M [1...

Ngày tải lên: 07/03/2014, 16:20

... were corroborated by a molecular model of the epitope and by immunization experiments with peptide-conjugates. Materials and methods Synthetic peptides Peptides were prepared by automated solid-phase ... reduction by dithiothreitol treatment. The yield of the oxidation reaction for most substitution analogues was between 65 and 75% and the three different isoforms we...

Ngày tải lên: 08/03/2014, 08:20

... composed by b-strands 145–150 and 156–161 and they are rich in hydrophobic and exposed residues. In particular, the aromatic rings of Y140 and W146 have high accessible surface area (30% and 35%, ... the membrane. Materials and methods Expression and purification of StnII-R29Q and StnII-Y111N mutants The unlabelled StnII-R29Q and the double uniformly labelled 13 C ⁄ 1...

Ngày tải lên: 14/03/2014, 23:20

... closing. [5]. This ‘tug- of- war’ scenario, whereby the closing of one active site exerts pressure through a suite of atomic interactions to inhibit the binding and closing of any other active sites ... essentially allowing only one of a set of interacting active sites to complete a catalytic cycle at a time. Further understanding of catalysis and the interaction of active...

Ngày tải lên: 16/03/2014, 06:20