Báo cáo khoa học " NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters " pdf

... NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters Umberto Tomati, a Monica Belardinelli, a Emanuela Galli, a Valentina Iori, a Donatella ... high-field NMR study of the polysaccharidic fraction extracted from Lentinula edodes mycelium grown on olive mill waste waters is reported....

Ngày tải lên: 28/06/2014, 11:20

... preventing the second reaction step (exon ligation). In addition, the product of the first reaction step is actually the intermediate in the full TES reaction. Substrate binding The rate constant for the ... 8) of the fraction product bound versus ribozyme concentration [32,33]. For the equilibrium dissociation constant of the substrate, K d S , an estimated value c...

Ngày tải lên: 18/02/2014, 18:20

... in the k cat for Cys. This implies that the hydroxyl group of Tyr108 of EhMGL1 is actively involved in the b-elimination and c-elimination of the C–S bond, but not the b-elimina- tion of the ... similar to TFM. Three fluorides on the methyl carbon of TFM and a sulfur atom of the thiol group of Hcy may participate in the formation of additional hydrogen bonds...

Ngày tải lên: 07/03/2014, 05:20

... oxidation of the substrate DHO with the quinone cosub- strate was coupled to the reduction of the chromogen 2,6-di- chloroindophenol. The K m of DHO was determined by varying the concentration of ... (1–1000 lm) at a fixed concentration (200 lm)ofQ D . The K m of Q D was determined by varying the concentration of Q D (0.1–200 lm) at a fixed concentration (1 m m) of...

Ngày tải lên: 07/03/2014, 12:20

... mammals, so the biosynthesis of PUFAs goes through the desaturation and elongation of essential FAs taken from the diet, linoleic and a-linolenic (18:3 n-3) acids, which allow the production of n-6 ... and from the alga Thal. pseudonana. Discussion We have functionally characterized all front-end desaturases detected in the genome databases of the three trypanosomati...

Ngày tải lên: 07/03/2014, 12:20

... 5.5, containing 1 mm CaCl 2, in the presence of 2.5 mm H 2 O 2 and varying concentrations of hydrogen donor, at 25 °C. The final enzyme concentration was 10 nm and the reaction was started by the ... In the presence of Ca 2+ , the T m of SPC4 is near that of soybean peroxidase, which is one of the most stable plant peroxidases with a T m of 90 °Cin the presence o...

Ngày tải lên: 16/03/2014, 13:20

... obtained by heterologous expres- sion in Escherichia c oli. The construction of the synthetic gene, e xpression c onditions, purification protocol and characterization of the recombinant protein will ... first- order c onditions, in w hich the c oncentration of p rotein acceptor, either in the direct reduction by 5-dRfH, or in the interprotein electron transfer, was in large...

Ngày tải lên: 16/03/2014, 16:20

... enaturin g con ditions (in the presence of 8 M urea); lane 6, elution 3 under denaturing conditions (in the presence of 8 M urea); lane 7, elution 4 under denaturing conditions (in the presence of 8 M urea); lane ... Interestingly, with the introduction of meth anol, the CD spectra of Nogo-40 undergo dramatic changes. The CD spectra of Nogo-40 in the presence of me...

Ngày tải lên: 16/03/2014, 16:20

... centrifugation of solubilized thylakoid membranes (fractions A–C, Fig. 1A). Fraction A in the upper part of the gradient contained 24% of total chlorophyll content and the rest of the chlorophyll ... height of about 9 nm is constant in all the projections. As the length s of the side-views c orrespond well with the length and width of particle in the top-view...

Ngày tải lên: 16/03/2014, 18:20

... recognition site on the extracellular domain. The second step will then be the deposition of the ligand into the TM pocket causing the conformation change of the receptor and triggering the coupling of ... conformation movement and defining the final conformation of the eLPs. These studies were limited to the three eLP domains. The conformation of the eLPs...

Ngày tải lên: 16/03/2014, 18:20