... The sampleswere ultracentrifuged and the sediments and supernatants obtained were analyzed. (A) lane 1, Pharmacia low molecular mass standards; lane 2, ostreolysin (noncentrifuged); lane 3, ... It has been speculated thatAa-Pri1, and similar proteins, may have important roles inthe initial phase of fungal fruiting, such as hyphae aggre-gation [4], or in apoptosis [1]. Their exact ... ostreolysin was assayed using HT 1080(human fibrosarcoma cells) and MCF 7 (human breastadenocarcinoma cells). After thawing, cells were grown for a week in Eagle’s minimum essential medium, supplementedwith...