... seventhhistidine, adjacent to the sixth histidine involved in copper binding. Preceding the sixth histidine(H3Baccording to the nomenclature used in [6]), at theend of the CuB-binding site, tyrosinase-like ... oxidation as shown in Fig. 7B.Binding of monophenols to resting met-tyrosinaseresults in the inactive dead-end complex, but bindingof o-diphenols leads the enzyme to the oxy-tyrosinaseform, the ... cellculture media and enzymatic determinations were obtainedfrom Sigma Co. (St Louis, MO, USA).Protein determinationProtein concentration was determined with the bicinchoninicacid assay. Alternatively,...