TUMOR MICROENVIRONMENT AND MYELOMONOCYTIC CELLS pptx

... 1 Myelomonocytic Cells – Phenotypic and Functional Diversity in Tumor Microenvironment 1 Chapter 1 Cell Lineage Commitment and Tumor Microenvironment as Determinants for Tumor- Associated Myelomonocytic ... Part 1 Myelomonocytic Cells – Phenotypic and Functional Diversity in Tumor Microenvironment Tumor Microenvironment and Myelomonocytic Cells...

Ngày tải lên: 27/06/2014, 09:20

... 1 Myelomonocytic Cells – Phenotypic and Functional Diversity in Tumor Microenvironment 1 Chapter 1 Cell Lineage Commitment and Tumor Microenvironment as Determinants for Tumor- Associated Myelomonocytic ... secreted factors by tumor- infiltrating monocytes and tumor cells help modulate tumor growth and monocyte infiltration and fate determination contributin...

Ngày tải lên: 30/03/2014, 19:20

... 1 Myelomonocytic Cells – Phenotypic and Functional Diversity in Tumor Microenvironment 1 Chapter 1 Cell Lineage Commitment and Tumor Microenvironment as Determinants for Tumor- Associated Myelomonocytic ... features of myelomonocytic cells and details the general functions and the diversity of these cells in the tumor microenvironment; Section 2 focusses on...

Ngày tải lên: 30/03/2014, 22:20

... internalization and degradation [7]. Part 1 Breast Cancer Cell Lines, Tumor Classification, In Vitro Cancer Models Breast Cancer – Focusing Tumor Microenvironment, Stem Cells and Metastasis ... Buehring and Capes-Davis, have also documented contamination with HeLa and other cell lines, including cross-species Breast Cancer – Focusing Tumor Microenvironment, Stem...

Ngày tải lên: 27/06/2014, 17:20

... examined. In light of the data in the literature and our work with HepG2 cells, we concluded that pyruvate might act similarly in tumor cells and nontumor cells. It could then act as a protective agent ... of HepG2 cells during hypoxia and after reoxygenation. HepG2 cells were incubated for 6 h under normoxic and hypoxic conditions in the absence and presence of pyruvate (...

Ngày tải lên: 18/02/2014, 16:20

... 5¢-GCCACCCATTCGTCACAATC-3¢; and ON361, 5¢-ATGCAGAAGGGGATCCGG-3¢. Direct measurements of [Ca 2+ ] i in single cells Non-transfected HEK293 cells and HEK293 cells stably transfected with the 5-HT 7(b) receptor (KB1 cells) ... (HEK)293 cells expressing the 5-HT 7(b) receptor, KB1 cells, were cultured, washed and loaded with 5 l M FURA-2-AM for 20 min. The fluorescence intensity in...

Ngày tải lên: 19/02/2014, 18:20

... total cellular and chromatin-bound PCNA T24 were grown and incubated on coverslips. Two sets of hypoxic cells and of cells reoxygenated for 10 min and 30 min were prepared. One set of cells was directly ... incu- bation conditions, cells were trypsinized, fixed and stained as described in the Materials and methods. (A) Cells after medium renewal; (B) cells after medium...

Ngày tải lên: 21/02/2014, 00:20

... hydrochloride, 15 m M NaN 3 and 10 m M EDTA) per 6 · 10 7 cells, for analysis in ELISA and Western blot experiments. Another portion of the cells was spun down to object glasses and used for immunocyto- chemical ... in U937 cells shows a four-band pattern at SDS/PAGE (Fig. 1). At comparison with insect cell produced recom- binant cystatin F (Fig. 1, lanes 1 and 2), which appears...

Ngày tải lên: 21/02/2014, 01:21

... Sertoli cells are bFGF pr oducing c ells [2,33] and are the t arget of this growth factor as bFGF inhibits FSH-induced estradiol synthesis ([5], and our results). HSPG and especially glypicans and ... representative of t hree, ®ve and three dierent experiments (mean  SE) for 10-, 20- and 3 0-d ay-old-rat Sertoli cells, respectively . Ó FEBS 2002 HSPG, target and partners of...

Ngày tải lên: 21/02/2014, 03:20

... of a1-PTH and PIN-a on NG108-15 cells. In (A) and (B), the cells were stained with 2 l M FM1-43 dye for 30 min, and thereafter abundantly washed with dye-free solution before imaging and the addition ... control cells, and with cells treated with PIN-a, significant changes in fila- mentous actin immunolabelling distribution were observed in cells treated with a1-PTH (Fig. 9C)....

Ngày tải lên: 07/03/2014, 12:20