... cycling conditions: 98°C for one 15 second(sec) cycle and then 35 repeated cycles of 98°C for 5 sec,59°C for 10 sec, and 72°C for 15 sec, followed by a finalextension at 72°C for 5 minutes (min). ... cycling conditions: 98°C for one 30 sec cycle and then 35 repeated cycles of 98°C for 10 sec, 59°C for 15 sec, and 72°C for 30 sec, followedby a final extension at 72°C for 5 min.Table 3 outlines ... 2 cellstransformed with vectors pMAL-c2x:GP1 and pMAL-c2x:GP2, respectively (Figures 2 and 3). Specifically, ~98-, 63-, and 65-kDa proteins were detected for MBP-NP, -GP1-, and GP2-fusion...