Advances in Applied Biotechnology Part 11 pptx

... inactivate bacterial chromosomal genes (Dabert and Smith, 1997; Advances in Applied Biotechnology 198 pKD46 Host strain pKD46 Host strain Host strain 7. Dual antibiotic selection 6. Incubation5. ... host strain containing the plasmid of pKD46, encoding the recombinase. Electroporation is crucial. • Culturing in the rich medium (SOC) with 1 mM of arabinose, at 37˚C for 2 h....

Ngày tải lên: 22/06/2014, 03:20

... resulting in a substantially increase in recombinant protein yield, the protein yield was still considerably lower than the yield observed in E. coli BL21. This difference in recombinant Increasing ... Biotechnology Progress, in press Waegeman, H.& Soetaert, W. (2 011) . Increasing recombinant protein production in Escherichia coli through metabolic and genetic engineer...

Ngày tải lên: 22/06/2014, 03:20

... rise to a fast-growing multidisciplinary field that integrates knowledge of engineering, biology and medicine. Tissue engineering (TE) is a promising technology for overcoming the organ transplantation ... this work Advances in Applied Biotechnology 238 nanoparticles – the use of magnetic force for tissue engineering, termed “magnetic force- based Tissue Engineering (Mag-TE).”...

Ngày tải lên: 22/06/2014, 03:20

... compound-ring resonator in which a dual-coupler fiber ring is inserted into the main cavity. When combined in tandem with a mode-restricting intracavity tunable bandpass filter, the compound-ring ... controller. 3. Gain medium 3.1 Single gain medium Many gain media are suitable for single-wavelength lasing in a fiber cavity, including erbium-doped fiber amplifiers (EDFA) (Antoine...

Ngày tải lên: 19/06/2014, 23:20

... is 304 Advances in Analog Circuitsi Algorithm 2: Modified Branch and Bound(d inc ,D N , ˆ D N rel ) Input: incumbent d inc , parameter domain D N , relaxed domain ˆ D N rel 1if d inc solves the sizing ... is found is in general non-optimal in terms of the objective function. Taking into account that it is a binary decision if a certain point solves the sizing task or not, the branching...

Ngày tải lên: 20/06/2014, 06:20

... Andronescu, A.(2 011) . Enhanced Cultivation of Edible and Medicinal Mushrooms on Organic Wastes from Wine Making Industry. In: Proceedings of the International Conference „Environmental Engineering and ... important biomass resources in residues from agriculture and forestry including both wet and dry waste Advances in Applied Biotechnology 22 SimpBTH 2009, Biotechnology...

Ngày tải lên: 22/06/2014, 03:20

... objective of increasing sustainability in PHAs synthesis processes. 2.2.1 Modification of host strains When they are grown in bioreactors, all microorganisms, including PHB-producing recombinant ... haemoglobin is supposed to facilitate intracellular oxygen transfer and assimilation, and the gene encoding this protein was introduced in PHB-producing E. coli improving the growth an...

Ngày tải lên: 22/06/2014, 03:20

... dealing with the use of bacteriocins or bacteriocin-producing strains for the biopreservation and improving functional properties of vegetable and fruit products. Advances in Applied Biotechnology ... 2000). Advances in Applied Biotechnology 66 bacteriocin is produced by the GRAS microorganism Lactococcus lactis and is used as a food additive in at least 48 countri...

Ngày tải lên: 22/06/2014, 03:20

... tyrosine- and putrescine-degrading enzymatic activities of wine- Advances in Applied Biotechnology 80 Although the primary goal is the prevention of mycotoxin contamination, mycotoxin formation ... histamine, tyramine and/or putrescine degradation among wine LAB does not appear to be very frequent, since out of the 85 strains examined, only nine displayed noteworthy amine-degra...

Ngày tải lên: 22/06/2014, 03:20

... naringin, rutin, quercetin and naringenin content Igual et al., 2 011 Bean (Quercetin , kaempferol) Atmospheric (100°C) and pressure boiling (121°C) with and without soaking and draining ... without draining stages induces 1-90% losses of quercetin and kaempferol in Brazilien beans (Ranilla et al., 2009). Advances in Applied Biotechnology 116 Food product Processi...

Ngày tải lên: 22/06/2014, 03:20