... were prepared by the reaction of the activatedfolate ester with the amine group on the PEAs.Evaluation of cytotoxicity of three PEI-derived materialsThe cytotoxicity of the three modified PEI-derivedmaterials ... Gou M, Dai M, Li X, Cao M, Huang M, Wen Y, Kan B, Qian Z, Wei Y:Preparation, characterization, and in vitro cytotoxicity study of cationicPCL-pluronic-PCL (PCFC) nanoparticles for gene delivery. ... ratio =7.53 × weight ratio of PEI/DNA [10]. Where N i s thenumber of polymer nitrogen at oms and P is the number of DNA phosphorus atoms. As controls, same quantity(1:1) of 25-kD PEI and GFP was...