Báo cáo hóa học: "Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168" pdf

... thermostable PHY US417 phytase from B. subtilis US417 in B. subtilis 168. Accordingly, the phytase- encoding gene was cloned in the pMSP3535 vector, and then, for the first time for a plasmid carrying the ... plate counting. Evaluation of culture conditions affecting phytase production by the recombinant Bacillus The factors affecting recombinant...

Ngày tải lên: 21/06/2014, 19:20

... (2008) to determine the first amino acid of the mature rPHY US417. The temperature profile of rPHY US417 was obtained by determining its activity between 37 and 80 °C at pH 7.5. Thermostability ... for overproduction of recombinant proteins in Bacillus is highly promising. 2 Abstract To attempt cost-effective production of US417 phytase in Bacillus...

Ngày tải lên: 20/06/2014, 20:20

... allowing the interaction of Antarctic b-tubulin isotypes with CCT in the adverse energetic conditions of the Antarctic habitat. The role of these amino acid substitutions in the folding process of E. ... analysis of the interaction between CCT and b-tubulin by cryoelec- tron microscopy allowed the identification of three regions from the N-terminal, and fiv...

Ngày tải lên: 23/03/2014, 21:20

... peptides carrying the HLA-A24-binding motif were synthesized from the amino acid sequence of this protein and analyzed to determine their binding affinities to HLA-A24. By stimulating patient's ... killing assays and drafted the manuscript. TT and YH participated in the design of the study and performed the evaluation of the data. TT helped to draft the ma...

Ngày tải lên: 18/06/2014, 15:20

... immunization) of EAE show extensive inflammation involving the white and grey matter (D), with marked demyelination in the inflammatory areas (F). The majority of the infiltrating inflammatory ... apoptosis of infiltrating cells in the CNS of animals with EAE [80]. The limitation of inflammation seen in the CNS could also be the result of a diminished anti...

Ngày tải lên: 19/06/2014, 22:20

... analysis on the OV90 drug sensitive cell line and on the resistant cell lines using Illumina Sentrix microarrays. For each of the resistance types (cisplatin, doxorubicin, and paclitaxel) two independent ... doxorubicin. The combination of a platinum compound (cisplatin) and paclitaxel represent the standard initial chemotherapy for ovarian cancer, while doxorubicin ha...

Ngày tải lên: 20/06/2014, 08:20

... means of interpreting the pairs (i, j) of operators included in the relation as the graph edge s. It should be noted that the conflict graph configuration and the accuracy of the final pipeline ... implementations. In order to overcome these issues in the state of the art of high-level modeling and synthesis, the Ptolemy project at the University of C...

Ngày tải lên: 20/06/2014, 22:20

... quality and protein engineering studies are possible for the first time. The purification using the N-terminal His-tag via nickel based affinity chromatography turned out to be efficient and fast. While ... molecular weight of 42.9 kD of the His-tagged protein. After successful recombinant expression, a nickel- based affinity chromatography of the His-tagged protein...

Ngày tải lên: 21/06/2014, 02:20

... 18. Description of the instruments and variables The Spanish versions of the VSP-A and KINDL-R were administered together with other demographic and health status variables. The VSP-A and the KINDL-R ... for further application in clinical practice and health service research the meaning of their scorings should be studied in depth. The aim of this study wa...

Ngày tải lên: 18/06/2014, 18:20

... excised and the DNA was extracted using the Qiaquick gel extraction kit (Qiagen). DNA was eluted off in a final volume of 20 µl elution buffer. The DNA was cloned into the TOPO cloning vector (Invitrogen) ... were grown in LB broth overnight and the DNA was isolated according to the manufacturer's protocol (Qiagen) eluting the DNA off in a final volume of 25...

Ngày tải lên: 20/06/2014, 01:20