... control. For trans- fection, cells were plated in 10 cm dishes at 50 % conflu- ency, and siRNA duplexes (50 nm) were introduced into the cells using Lipofectamine 2000 (Invitrogen) according to the ... followed by a final resuspension in lysis buffer ( 150 mm NaCl, 50 mm Tris ⁄ Cl, pH 7.4, 1% NP-40, 0. 25% sodium deoxycholate, 1mm EGTA) containing protease inhibitors for weste...
Ngày tải lên: 07/03/2014, 05:20
... develop the habit of reviewing your e-mail at certain intervals in the day—first thing in the morning, mid-morning, before leaving for lunch, mid-afternoon, and prior to leaving for the day. Don’t leave ... ground. Other E-Organizing Tips • Post your rules for interruption-free zones. • Set clear guidelines around electronic devices in meetings. • Use signatures for more than j...
Ngày tải lên: 24/01/2014, 09:20
Unit9 : ACTIVITIES FOR NEXT SUNDAY
... … . Alan: What are you going to do tomorrow? Nam:I’m going to play football. What about you, Alan? Alan:I’m going to visit Ha long Bay. Activities for next Sunday Unit9: Wednesday,February ... Vocabulary Vocabulary Activities for next Sunday Unit9: Wednesday,February 18th 2009 Lesson one: section A1,2,3 III. Practice: I They A:What ………going to do ……? B:……… going to...
Ngày tải lên: 20/07/2013, 01:28
101 activities for teaching creativity and problem solving
... 221 52 . Turn Around 2 25 Just Alike Only Different Activities 53 . Bionic Ideas 229 54 . Chain Alike 234 55 . I Like It Like That 238 56 . What Is It? 241 Group Only Activities 2 45 Chapter 9: Brainstorming ... Brainwriting is a term coined in Germany that refers to the silent, written generation of ideas in a group setting. Brainstorming and Brainwriting All things bein...
Ngày tải lên: 12/08/2013, 20:09
22 FUN activities for kids 22 hoạt động vui nhộn dành cho trẻ
... Continue draping the remaining ones using the chairs. Tying the ends around a doorknob or tucking it under another piece of furniture will add more room. 3. When the Tent City is up, play inside ... coordination will also increase. material needed: let's get started: 1. Place the paper on a flat surface and get comfortable. drawing paper, colored pencils DESIGNER SHOP Draw outfit...
Ngày tải lên: 17/08/2013, 08:17
Microsoft office excel 2007 for project managers
... the Initiating Process 51 The Elements of a Project Request Form 51 Establishing the Business Justification 54 Creating the Project Request Form using Excel 56 Publishing the Project Request Form ... Control Forms 250 Change Request Form 250 Change Request Log 251 Converting Tables to Ranges 253 Reporting and Printing Change Control Data 254 Assessing the Impacts of Change 26...
Ngày tải lên: 22/08/2013, 13:34
ACTIVITIES FOR SPEAKING.doc
... dialogue, they can practice again, using information of their own. 8. Dialogue creation. Students can be, given the beginning lines in a dialogue and instructed to finish the dialogue creatively. 9. ... am studying English now. 6. Chaining. In this kind of exercise, students are given a simple dialogue in which they practice a given pattern. Example: Minh:Hi. I’m Minh Mai: Hello. Minh....
Ngày tải lên: 28/08/2013, 11:10