... resulting clones weregrown, induced for expression of IgG, and subjected to anadditional round of sorting.Screening of selected clones by ELISAFollowing two rounds of FACS selection, single ... through in- line flow cells at a flow rate of 50 lLÆmin)1(5 min of sta-bilization, 1 min of association, and 5 min of dissociation), and the surface was regenerated using two pulses of 100 mmH3PO4. ... fluorescent cells falling into the double-positive quadrant, indicating both expression and affinity,were sorted on a FACSAria droplet deflection flow cytome-ter (Becton Dickinson, Franklin Lakes, NJ,...