... 25:3639-3644.18. Katagiri T, Yamaguchi A, Komaki M, Abe E, Takahashi N, Ikeda T,Rosen V, Wozney JM, Fujisawa-Sehara A, Suda T: Bone morphoge-netic protein-2 converts the differentiation pathway of C2C12 ... cell activity assay (alamarBlue, Assay, Biozol,Eching, Germany). For the assay, 10% alamarBlue wasadded to the cells and incubated for 3 h at 37°C. Theabsorbance was measured in triplicate ... isimportant [14]. Once a growth factor reaches the site of action, it must remain at the site in an appropriate con-centration and long enough for the pharmacologicaleffect. The half life of growth...