báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx

... the design of the study and performed the evaluation of the data. TT helped to draft the manuscript. YH contributed to the HLA-A24-binding assay and CTL induction from PBMCs. HK, ES and NM contributed ... indicating the adequate qualification of this assay. The HLA-A24 level on the cell surface of T2-A*2402 cells was up-regu- lated in the presence of AMAC...

Ngày tải lên: 18/06/2014, 15:20

... emerged from Finland, suggesting that the lack of CCR5 does not protect from MS, but rather it may predispose to the chronic course of the disease [69]. This would further imply that in view of the ... purposes) macrophages and resident microglia to sites of CNS inflammation. Detailed knowledge of expression patterns is crucial for the understanding of therapeuti...

Ngày tải lên: 19/06/2014, 22:20

... following the development of drug resistance. A. Heat map showing the expression of all the significant genes analyzed using the Illumina bead array (845 genes). Changes in gene expression for the ... to the parental OV90 line. B. Multi-dimensional scaling plot indicating the cell lines used for the gene expression profiling analysis. Each of the two different...

Ngày tải lên: 20/06/2014, 08:20

... of several pathologies and because of the potential use of the toxins as biological weapons. Alteration of their MHC and TCR binding capacity by site directed mutagenesis could be useful in the ... two bands of protein were observed in SDS/PAGE (Fig. 1A). The weaker band has an apparent molecular mass in agree- ment with the theoretical value calculated from the am...

Ngày tải lên: 07/03/2014, 16:20

... regulate the biosynthesis of the lipopeptides. The experiments performed in the present study showed that this AAT can form Glu and Asp, and the forma- tion of Glu and Asp is used to synthesize Gln and Asn, ... determine the exact role of three residues in AATB3. The loss of the activity from the mutations together with the multiple alignment analysis ind...

Ngày tải lên: 14/03/2014, 23:20

... levels in the m edial striatum of a ny of the w ild-type and HD mice examined. The optical density of the hybridization signal in the lateral striatum, corrected by subtracting the signal in the m ... length of the CAG repeat and relative expression of the human HD transgene in the two strains of mice and that mutant huntingtin differentially affected C...

Ngày tải lên: 16/03/2014, 18:20

... way except for the presence of the linker and the family-2 CBM at the C terminus of XEG74. The molecular masses of Cel9A-68 (CD + family3c CBM) and Cel48ACD, both cloned with the Cel6A signal ... glucose standard curve and the nmoles of XGOs produced was determined for both the PAHBAH and the bicinchoninic acid reducing sugar methods. For the PAHBAH method,...

Ngày tải lên: 23/03/2014, 21:20

... volume. Nitrite released from the reaction was determined by the method of Little [28]. One unit of NaoA is defined as the amount of enzyme required to catalyze the formation of 1 lmol nitriteÆmin )1 . ... to the experiment to identify whether the protein encoded by the complete DNA fragment can catalyze the oxidation of nitroalkanes. In this paper, we describe the...

Ngày tải lên: 31/03/2014, 08:20

... −xy), and the proof of the first part of Theorem 3.6 is complete. The proof of the second part is similar. Note that the condition c ∈ (1/2, ∞), d ≥ c/(2c −1) of Lemma 2.11 is equivalent to the ... parts (1) and (4) of Lemma 2.11 give R −B + 1 < 0 and B − 1 < 0. Because the right-hand inequality is a consequence of Theorem 3.6, part (2), the proof is com...

Ngày tải lên: 18/06/2014, 15:20

... higher than 3.5 kg. The expression of specific endothelial cell markers (von Willebrand factor, CD31, and the binding and internalization of acetylated low-density lipoprotein) and the smooth muscle ... cell cultures and carried out the molecular and cellular analy- sis. CGV and JLF informed the parents about the objec- tives of the research project, did the...

Ngày tải lên: 18/06/2014, 15:20