... USA) using the DNA thermalcycler. After 3 min denaturation at 94°C, 30 PCR cycleswere performed, each cycle consisting of 94°C for 1.5min, 65°C for 1 min and 72°C for 1 min, and a final 6 minelongation ... sjTRECs. We showed a prominent decrease of sjTRECs levels in CML, indicating the reduction of recentthymic emigrants affects the majority of TRBV subfami-lies.Competing interestsThe authors ... Guangzhou, 510632, China, 2Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China, 3Department of Hematology, Guangdong Province People's...