... ovary and oviduct.DiscussionIn the present study, out of 420 organ samples tested, 75% of spleen, 68.3% of bone marrow, 70% of thymus, 53.3% of liver, 46.7% of duodenum, 66.7% of ovary and 48.3% of ... volume of 25 μl using the followingcycling parameters: initial denaturation of 94°C for 2 minfollowed by 35 cycles of denaturation, annealing and extension at 94°C for 2 min, 50°C for 1 min and ... isolated and characterized. Detection of CAV positive embryos by the PCR assay in the range of 40 to 100% for different farms indicated high level of occurrence of vertical transmission of viral...