... in this s tudy excludes the idea of a c atalytictriad involved in C-domain catalysis of NRPSs. With the®nding of the additional residue R62 and con®rming theresidues H147 and D151 abolishing ... systems of gramicidin S (Grs), tyrocidin A (Tyc), s urfactin (Srf),lichenysin (Lic), bacitracin ( Bac), fengycin (Fen), ente ro-bactin (Ent), chloroeremomycin (Cep), pristinamycin (Snb),enniatin ... demonstrate that C-domainspossess an in trinsic editing function for the incomingaminoacyl moiety [6]. By using aminoacyl-S-CoA as probesit was shown that the ®rst C-domain of the tyrocidinesynthetase...