... oligonucleotides, 5¢-GATGAATTCGGAGGTTTAAATTTATGTACCAAC CTGTCGCTCTATTTA-3¢ and 5¢-CAATTCAAGCTTAA TGATGATGATGATGATGCTCCAGTTCATAACGTAA AGCCTCAGCGG-3¢. The amplified DNA was digested with Eco RI and HindIII, and then ... of pJY310 [7] was amplified by PCR using a pair of oligonu- cleotides, 5¢-GAGCTCGAAGGAGATATAAATATGAAT AAGATCCTGTTGCAATGC-3¢ and 5¢-AAGCCTGCAG TTTTTGTTCCACCAATATCAAACCC-3¢. Th...
Ngày tải lên: 19/02/2014, 00:20
... GCTTTTAAAGTTGACTTCAAAG 2 CTTTGAAGTCAACTTTAAAAGC 3 CTA CCATGGCACCTCCTTCTTCTTTCTCAA 4 GCT GTCGACTTATTTAGTGCATGCTTTATAAACAA 5 CTA CCATGGCCCCCATCTCTTTTAGTCAT 6 GCT GTCGACTCAGTTCGGGCATTGCTCAC B. Altermark ... and the mean values are drawn. AB Fig. 8. Cleavage of plasmid, dsDNA and ssDNA. (A) 14 nM VcEndA incubated at 23 °C for 5 min with plasmid (lane 2), dsDNA (lane 4) and ssDNA (lane 6). Subst...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: "The impact of language models and loss functions on repair disfluency detection" pptx
... noisy- channel model. We show that language mod- els trained on large amounts of non-speech data improve performance more than a lan- guage model trained on a more modest amount of speech data, and ... a similar formula. 9 Results We follow Charniak and Johnson (2001) and split the corpus into main training data, held-out train- ing data and test data as follows: main training con-...
Ngày tải lên: 20/02/2014, 04:20
Tài liệu Báo cáo khoa học: "Automatic Evaluation of Machine Translation Quality Using Longest Common Subsequence and Skip-Bigram Statistics" doc
... and human perform- ance and the reference translations are usually the only human translations available. Using this pro- cedure, we are able to estimate average human per- formance by averaging ... NIST. 2002. Automatic Evaluation of Machine Translation Quality using N-gram Co- Occurrence Statistics. AAAAAAAAAAA http://www.nist.gov/speech/tests/mt /doc/ ngram- study.pdf Pantel,...
Ngày tải lên: 20/02/2014, 16:20
Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
... Katayama Y, Hiraishi A & Kuraishi H (1995) Para- coccus thiocyanatus a new species of thiocyanate utiliz- ing facultative chemolithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus ... diffraction data of the wt and M100K crystals were collected on an in-house beam using a MAR345 Image Plate detector. The crystals were mounted in a capil- lary and datas...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo Y học: Functional assignment of motifs conserved in b1,3-glycosyltransferases A mutagenesis study of murine UDP-galactose:b-N-acetylglucosamine b1,3-galactosyltransferase-I pptx
... TGCTTCATCTTG CTGACGTGTACGTGGGACT C27 1A ATGTGTACGTGGGACTGGCACTTCGAAAGC C29 5A AAAATGGCCTACAGTTTA GCTCGGTACC W31 5A CAGAATC GCCAATGACATGTCAAGGAAGAAGCATCTGAGATGTTAGTCTAGATAT C32 6A GTCAAGGAAGAAGCATCTGAGA GCCTAGTCTAGATAT 234 ... AAATGAGCCCAACAAAG CCGAGAAAAACATT I9 7A- R9 8A AATTTGATGCTCGACAGGCT GCCGCGGAGACATGG W10 1A CAATCCGGGAGACA GCTGGTGATGAAAA F11 6A- L11 7A- L11 8A- G11 9A TAGCCACACTT...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
... (F) (5¢-GACTGCGCCCAGGTGTTCCAGAATGAGAAG-3¢) and (R) (5¢-CTTCTCATTCTGGAACACCTGGGCGCAG TC-3¢); AKR1B3 H110F (F) (5¢-GATCTCTACCTTATT TTCTGGCCAACGGGG-3¢) and (R) (5¢-CCCCGTTGGC CAGAAAATAAGGTAGAGATC-3¢) (the italic codons indicate ... (5¢-CCTCTACCTTATTTTCTGGCCGACT GGC-3¢) and (R) (5¢-GCCAGTCGGCCAGAAAATAAG GTAGAGG-3¢); AKR1B1 H11 0A (F) (5¢-CCTCTACCTT ATTGCCTGGCCGACTGGC-3¢) and (R) (5¢-GCCAGTC- GGC...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Systemic RNAi of the cockroach vitellogenin receptor results in a phenotype similar to that of the Drosophila yolkless mutant ppt
... D. melanogaster (AAB60217), A. gamb- iae (EAA06264), A. aegypti (AAK15810), S. invicta (AAP92450) and P. americana (BAC02725), and the verte- brates Anguila japonica (BAB64337), Conger myriaster (BAB64338), ... different developmental stages using the General Elute Mammalian TotalRNA kit (Sigma, Madrid, Spain). A 300 ng portion of each RNA extraction was DNAse treated (Promega, Madi- so...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation potx
... Haruki*, Kazufumi Takano, Masaaki Morikawa and Shigenori Kanaya Department of Material and Life Science, Graduate School of Engineering, Osaka University, Japan A psychrotrophic bacterium Shewanella ... the NdeI–BamHI sites of pET-2 8a. This DNA fragment was amplified by PCR. The sequences of the PCR primers were 5¢-AGAGAGAATT CATATGTCAGATTTGTTCAG-3¢ for the 5¢-primer and 5¢-GGCCACT...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Critical roles of conserved carboxylic acid residues in pigeon cytosolic NADP+-dependent malic enzyme docx
... decarboxy- lation activity of malic enzyme was assayed by the method of Tang & Hsu [38] using oxaloacetate as substrate. The rate of decarboxylation of oxaloacetate was measured by monitoring the disappearance ... (QuantumSoft, Uetikon am See, Switzerland). Partial reaction analysis The two partial activities of malic enzyme, decarboxylation and reduction, can be evaluated separ...
Ngày tải lên: 23/03/2014, 10:21