... sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses. Table 1: PCR ... a novel RV2 rhadinovirus in the baboon, Papio cynocephalus, using the RV2 QPCR assay To further determine the specificity of the RV2 QPCR assay, DNA obtained from lympho...
Ngày tải lên: 20/06/2014, 04:20
... [24] Determination of the level of RNA products of the H19 gene The PCR reactions were carried out in 25 μl volumes in the presence of 6 ng/μl of each of the forward and the reverse primers using 0.05 ... participated in the analyses of the ovarian ascites fluid. SA participated in the in vitro studies. JG participated in the in vitro studies....
Ngày tải lên: 18/06/2014, 15:20
Báo cáo hóa học: " Development of targeted therapy for bladder cancer mediated by a double promoter plasmid expressing diphtheria toxin under the control of H19 and IGF2-P4 regulatory sequences" potx
... was designed to bind the 3’ end of exon 7 and the 5’ end of exon 8 without the introns in betwe en. The integrity of the cDNA was assayed by PCR analysis of the ubiquitous, cell cycle independent, ... known autocrine/paracrine mode of IGF2 mitogen action in the development of a wide range of human malignancies. Accordingly, destruction of the H19 and IGF2...
Ngày tải lên: 18/06/2014, 16:20
Báo cáo hóa học: "Involvement of aryl hydrocarbon receptor signaling in the development of small cell lung cancer induced by HPV E6/E7 oncoproteins" ppt
... release the E2F transcription factor, subsequently leading to progression of the cell into the S-phase [14]. Furthermore, E7 binds to inhibitors of cyclin-dependent kinases (p16, p21), increasing the ... to neurogenesis. The list of these genes is reported in addi- tional file 2. These results support the hypothesis of a possible role of E6 and E7 in the induction...
Ngày tải lên: 18/06/2014, 16:20
báo cáo hóa học: " Development of the Knee Quality of Life (KQoL-26) 26-item questionnaire: data quality, reliability, validity and responsiveness" pptx
... patients stating that there had been no change in their knee condi- tion in the two weeks between administrations were included in the test-retest analysis. None of the test-retest studies reported in the ... for exist- ing instruments. In common with the majority of these instruments, the highest correlations were found between the KQoL-26 and the SF-36 scale o...
Ngày tải lên: 18/06/2014, 19:20
Báo cáo hóa học: " Development of a parent version of the Manchester-Minneapolis quality of life survey for use by parents and carers of UK children: MMQL-UK (PF)" potx
... through- out the study, was involved in the final study analysis and was involved in writing the manuscript. AM provided clin- ical support for the study, was involved in designing the study and was involved ... involved in writing the manuscript. CE was involved in designing the study, provided methodological support throughout and was involved in writing the man-...
Ngày tải lên: 18/06/2014, 22:20
Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... and the membrane was removed from the min- iblotter. The remaining active esters on the membrane were hydrolyzed by incubation in 0.1 M NaOH for 8 min at room temperature and rinsed in water. The ... were used in the following RT-PCR assay at a final volume of 50 µl. The reaction contained 4 µl of cDNA from the RT reaction, 5 µl of 10× PCR Buffer, 1.5 mM MgCl 2 , 1...
Ngày tải lên: 20/06/2014, 01:20
báo cáo hóa học:" Development of the Well-being questionnaire short-form in Japanese: the W-BQ12" pptx
... measures of depression and anxiety, the mean score of the Negative Well-being subscale was sig- nificantly higher in women, indicating more negative well-being in women than in men. Insulin-treated patients ... support for combining all items into a sin- gle total General Well-being score. The factor structure found in the total sample was similar within the two se...
Ngày tải lên: 20/06/2014, 15:20
báo cáo hóa học:" Development of a patient reported outcome measure for fatigue in Motor Neurone Disease: The Neurological Fatigue Index (NFI-MND)." pot
... difficulties of administering a suite of questionnaires, including a 52-item fatigue measure, in a short clinic appointment. Many patients expressed a preference to take the pack home to complete. The ... good indicator of the levels of fatigue displayed by the respondents. Whenever parametric statistics are required for the data, the ordinal-interval conversion can...
Ngày tải lên: 20/06/2014, 15:20
báo cáo hóa học:" Development of the Japanese version of the Pediatric Quality of Life Inventory™ Brain Tumor Module" pot
... lifestyle of the child had changed since responding to the initial questionnaire. We therefore analyzed the retest answers of the remaining 22 children and 27 parents. On comparison of characteris- tics ... excluded from the study if hospital doctors or social workers of the CCAJ determined the family to be unsuitable for participating in the study due to finding t...
Ngày tải lên: 20/06/2014, 16:20