báo cáo hóa học:" Development of the Well-being questionnaire short-form in Japanese: the W-BQ12" pptx

báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot

... sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses.Table 1: PCR ... a novel RV2 rhadinovirus in the baboon, Papio cynocephalus, using the RV2 QPCR assayTo further determine the specificity of the RV2 QPCRassay, DNA obtained from lymphocytes of baboonPcy78404 ... QPCRassays. While all of the samples were positive for the singlecopy OSM gene, only six of the thirty macaques werepositive for the presence of an RV2 rhadinovirus. In all of these six cases, both...

báo cáo hóa học:" Development of targeted therapy for ovarian cancer mediated by a plasmid expressing diphtheria toxin under the control of H19 regulatory sequences" doc

... [24]Determination of the level of RNA products of the H19 gene The PCR reactions were carried out in 25 μl volumes in the presence of 6 ng/μl of each of the forward and the reverseprimers using 0.05 ... participated in the analyses of the ovarian ascites fluid. SA participated in the in vitro studies. JG participated in the in vitro studies. IMparticipated in the in vivo studies. RA participated in the PCR ... signals. The quantity of the staining was referred to (+1) up to one third of the cells, (+2) one to two thirds of the cells and (+3) more than two thirds of cells (I-indicates the intensity of the...

Báo cáo hóa học: " Development of targeted therapy for bladder cancer mediated by a double promoter plasmid expressing diphtheria toxin under the control of H19 and IGF2-P4 regulatory sequences" potx

... wasdesigned to bind the 3’ end of exon 7 and the 5’ end of exon 8 without the introns in betwe en. The integrity of the cDNA was assayed by PCR analysis of the ubiquitous,cell cycle independent, ... knownautocrine/paracrine mode of IGF2 mitogen action in the development of a wide range of human malignancies.Accordingly, destruction of the H19 and IGF2 expres-sing tumor cells not only will eliminate part of ... promoters and therefore drive the expression of DTA within the cells and in consequence triggeringtheir necrosis.Finally, we dealt with the question whether transfec-tion of both single promoter...

Báo cáo hóa học: "Involvement of aryl hydrocarbon receptor signaling in the development of small cell lung cancer induced by HPV E6/E7 oncoproteins" ppt

... release the E2F transcription factor, subsequentlyleading to progression of the cell into the S-phase [14].Furthermore, E7 binds to inhibitors of cyclin-dependentkinases (p16, p21), increasing the ... toneurogenesis. The list of these genes is reported in addi-tional file 2. These results support the hypothesis of apossible role of E6 and E7 in the induction of neuroen-docrine differentiation of SCLC. ... Kinase. Evalua-tion of the results obtained in the two systems showedderegulation of the same pathways in human SCLC and in that induced experimentally by the E6/E7 oncopro-teins of HPV16.To furth...

báo cáo hóa học: " Development of the Knee Quality of Life (KQoL-26) 26-item questionnaire: data quality, reliability, validity and responsiveness" pptx

... patientsstating that there had been no change in their knee condi-tion in the two weeks between administrations wereincluded in the test-retest analysis. None of the test-reteststudies reported in the ... for exist-ing instruments. In common with the majority of theseinstruments, the highest correlations were found between the KQoL-26 and the SF-36 scale of physical functioning. The Edinburgh Knee ... majority of theseitems and items within the physical functioning compo-nent had higher loadings within the first factor of the unrotated solution and the majority had loadings over 0.4within the...

Báo cáo hóa học: " Development of a parent version of the Manchester-Minneapolis quality of life survey for use by parents and carers of UK children: MMQL-UK (PF)" potx

... through-out the study, was involved in the final study analysis andwas involved in writing the manuscript. AM provided clin-ical support for the study, was involved in designing the study and was involved ... involved in writing the manuscript. CE wasinvolved in designing the study, provided methodologicalsupport throughout and was involved in writing the man-uscript. SJ was involved in designing the ... report.Background The measurement of health-related quality of life (HRQL)is increasingly becoming part of the overall assessment of a patient's health both in the clinical and the research set-ting,...

Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt

... and the membrane was removed from the min-iblotter. The remaining active esters on the membranewere hydrolyzed by incubation in 0.1 M NaOH for 8 minat room temperature and rinsed in water. The ... were used in the following RT-PCR assay at a final volume of 50 µl. The reaction contained 4 µl of cDNA from the RT reaction,5 µl of 10× PCR Buffer, 1.5 mM MgCl2, 1 µl of 10 mMeach of dATP, ... samples from the 2006 out-break. Control samples containing known RT-PCR products from the reference panel are shown on the left binding to there respective probes. Clinical samples binding to probe...

báo cáo hóa học:" Development of the Well-being questionnaire short-form in Japanese: the W-BQ12" pptx

... measures of depression and anxiety, the mean score of the Negative Well-being subscale was sig-nificantly higher in women, indicating more negative well-being in women than in men. Insulin-treatedpatients ... support for combining all items into a sin-gle total General Well-being score. The factor structure found in the total sample was similarwithin the two sexes, and within the insulin- and tablet-treated ... beproblematic during cognitive debriefing. As there were noproblems in the understanding of the original 22 items, the five additional items were not used in the analysis.Psychometric evaluation of the...

báo cáo hóa học:" Development of a patient reported outcome measure for fatigue in Motor Neurone Disease: The Neurological Fatigue Index (NFI-MND)." pot

... difficulties of administering a suite of questionnaires, including a 52-item fatigue measure, in a short clinic appointment. Many patients expressed a preference to take the pack home to complete. The ... good indicator of the levels of fatigue displayed by the respondents. Whenever parametric statistics are required for the data, the ordinal-interval conversion can be employed, in the event there ... preventing such factors being a source of confounding effect in the phenomenon being measured. Item Category Thresholds The Rasch model also allows for a detailed analysis of the way in which...

báo cáo hóa học:" Development of the Japanese version of the Pediatric Quality of Life Inventory™ Brain Tumor Module" pot

... lifestyle of the child hadchanged since responding to the initial questionnaire. Wetherefore analyzed the retest answers of the remaining 22children and 27 parents. On comparison of characteris-tics ... excluded from the study if hospital doctors orsocial workers of the CCAJ determined the family to beunsuitable for participating in the study due to finding the subject of brain tumors too painful ... ceilings on the sample size. Instead, we seta ceiling of four months on the study duration. Poweranalysis using the findings from the original English ver-sion [16] demonstrated that the minimum...

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