... also thank the BBSRC for a PhDstudentship to J. G. B., the BHF for a PhD studentship to L. L. W., the MRC for the bioinformatics grant (64600017) and Dr ShahidulIslam for encouragement to undertake ... This may therefore account for the effects of2-APB on Ca2+binding. Also close to the drug in this site isPhe256, which is known to be important for the effects ofanother inhibitor, thapsigargin ... to the filter, to give the specific amount of Ca2+bound to the ATPase. A correction was also applied for nonspecificbinding of Ca2+ to the lipid [13].Modeling of the 2-APB-binding site on the...