... experiments, performed data analysis, and drafted the manuscript. VSC participated in the performance of the in vivo viralinfections and the isolation of CNS cells. MM participated in the preparationof ... release intact virions. The sonicated material wascentrifuged to remove unwanted cellular debris and the viral titers in the cell-free supernatant was quantifiedusing a standard plaque assay ... Cambridge, MA), STING (Abcam, Cambridge,MA) or a mouse monoclonal antibody against gG1(Abnova, Taipei, Taiwan) for 24 hours at 4°C, blots werewashed and incubated in the presence of an horseradishperoxidase...