báo cáo hóa học:" Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture" ppt

... Helsinki, Finland Email: Angelina Plyusnina - anguelina.pljusnina@helsinki.fi; Alexander Plyusnin* - alexander.plyusnin@helsinki.fi * Corresponding author Abstract Tula hantavirus carrying recombinant ... parental virus: analysis of consecutive passages in a cell culture Angelina Plyusnina and Alexander Plyusnin* Address: Haartman Institute, Department of Virology,...

Ngày tải lên: 20/06/2014, 04:20

... in this paper show that the recTULV presents no real match to the original cell adapted variant and that the lower fitness of the recombinant virus can not be increased by pre-passaging in cell ... weaker due to the fact that two non-canonical G:U base pairs presented in the plus-sense RNA occur as non- pairing C /A bases in the minus-sense RNA. Interestin...

Ngày tải lên: 18/06/2014, 22:20

... Public Health and Neuroscience, University of Pavia, Pavia, Italy Full list of author information is available at the end of the article Tonini et al . Journal of Occupational Medicine and Toxicology ... and interpreted the data. FM collected, analyzed and interpreted the data. CF collected, analyzed and interpreted the data, performed the statistical analysis. FS parti...

Ngày tải lên: 20/06/2014, 00:20

... F) is the relative frequency of codebook F in object instance l. We have to minimize intraclass variations. As mentioned, one of the main causes of large intraclass variation is surface markings, ... comparing visual appearance. In Figure 10, wheels in the car codebook and in airplane codebook have a similar appearance. At the same time, each category also has a...

Ngày tải lên: 21/06/2014, 08:20

... but seconding them, as in the case of additional provincial clinical care specialists, additional capacity remains external and limited to the period of GHI funding available. The more Zambia's ... of their clinic, imposing a further strain on the day -to- day running of the ART programme. Mentoring and the secondment of GHI staff to the public sector...

Ngày tải lên: 18/06/2014, 17:20

... icosahedron. The P domain is divided into sub- domains P1 and P2 that participate in dimeric contacts that increase the stability of the capsid. The P2 domain is an insertion in the P1 domain and ... 5'- GGG GAT ATC CAC CAT GGA GAC AGA CAC ACT CCT GCT AT -3' VK2-F 5'- GGG GAT ATC CAC CAT GGA TTT TCA AGT GCA GAT TTT CAG -3' VK3-F 5'- GGG GAT ATC CAC...

Ngày tải lên: 20/06/2014, 01:20

... made contribution in data analysis; CG made contribution to the analysis and interpretation of the data, in critical revision of the manuscript and in acquisition of funding. PDB conceived and designed ... the study, analysed and interpreted the data and drafted the manuscript. All authors read and approved the final manuscript. Authors’ information LP’s present...

Ngày tải lên: 20/06/2014, 03:20

... N-terminal His-tag to allow a functional expression of the 2,5-DKCMO in E. coli and easy protein purification by affinity chromatography. The N-terminal tag was favored compared to the C-terminal tag, ... (DBU, Osnabrück, Germany, Grant No. AZ13234) for financial support and Christin Peters and Ina Menyes for assistance in the laboratory. Competing interests The authors...

Ngày tải lên: 21/06/2014, 02:20

... respectively. The analysis of variance (ANOVA) tested using Fisher’s statistical analysis, was used to verify the adequacy of the model. The results are shown in Table 3. The model F- value of 99.32 ... activity of recombinant bromelain. An increase in recombinant bromelain production was seen by increasing the L-arabinose concentrations from 0.1−0.2 % (w/v...

Ngày tải lên: 21/06/2014, 17:20