Báo cáo hóa học: " Plaque assay for human coronavirus NL63 using human colon carcinoma cells" ppt

... flow cytometry was always >95% for NK cells and >98% for CD4 T cells and monocytes. Flow cytometry and cell surface staining Flow cytometry was performed using a LSR-II cytometer, BD biosciences, ... a RNAse solution for 30 min RT and washed with PBS containing 5% fetal calf serum before flow cytometry analysis. Equilibrium binding assay Cells in triplicates were incubated fo...

Ngày tải lên: 18/06/2014, 15:20

... species [52], therefore sorting o f human proteins might be different in a canine cell line. We therefore used human hepatoma HepG2 cells that polarize after several days in culture and form apical ... subsequently from human liver [11,15,16] and human tumor cells [17]. Trans- port studies using inside-out oriented membrane vesicles from liver [18,19] or from cells stably transfecte...

Ngày tải lên: 31/03/2014, 09:20

... [21]. This form is detected by immunohistochemistry in a wide vari- ety of human tissues that do not stain for noticeable amounts of fl-RAGE [22]. Over 20 different splice variants for human RAGE ... binding induces a large conformational change which exposes a hydro- phobic binding domain (except for S100A10 which is locked in this conformation) [47]. This change in confor- mation...

Ngày tải lên: 18/06/2014, 15:20

... year of formal education were doc- umented as demographic description for all respondents. For the population with diabetes, descriptive clinical data were also collected. Data analysis For each ... not for citation purposes) years) they would sacrifice in return for being relieved of the health state and going to perfect health. The higher the proportion the respondent is willing t...

Ngày tải lên: 18/06/2014, 19:20

... variable pair. Hypothesis testing for performance evaluation The formulation of the previous hypothesis test gives means for evaluating the whole classification chain per- formance. Receiver Operating ... the more effcient the classifier. Using an information theoretic framework based on [5] and [6], audio features specific to speech are extracted using the information content of both...

Ngày tải lên: 19/06/2014, 08:20

... custom cre- ated for each subject to ensure that the fingers are flexed to the same position for each test. The participant clenches the mold for 6 seconds and then releases the mold for 6 seconds. ... repeated 10 times. For each test above, the participant rested for at least 1 minute, and then repeated the entire test. This was done 10 times for both Test A and Test C, for a...

Ngày tải lên: 19/06/2014, 10:20

... with IL-4 or IL-13 for 24 hrs and the mRNA expression levels for AG1 (Panel A); MRC1 (Panel B); FIZZ and YM1 (Panel C) were determined using quantitative RT-PCR. mRNA levels for each of these ... 12 (page number not for citation purposes) vate; Invitrogen). Cells were then allowed to adapt to the serum-free media for 24 hours before starting the experi- ments. The stimulants use...

Ngày tải lên: 19/06/2014, 22:20

... encephalitis; ATCC, VR-539) at a low multiplicity of infection (MOI) of 0.05 plaque- forming units (PFU) per cell and incu- bated for 48 hours at 34°C in SFM4MegaVir protein- free medium (Thermo Scientific ... unwanted cellular debris and the viral titers in the cell-free supernatant was quantified using a standard plaque assay of serial dilutions of HSV- 1 on Vero cells at 34°C. MHV...

Ngày tải lên: 19/06/2014, 22:20

... algorithms. We give the first O(n 4) results for the natural formalism of bilexical context-free grammar, and for AI- shawi's (1996) head automaton grammars. For the usual case, split head automaton ... having the form A + a, where A E VN, a E (VN U VT)*. If every production in P has the form A -+ BC or A + a, for A,B,C E VN,a E VT, then the grammar is said to be in Chom...

Ngày tải lên: 20/02/2014, 19:20

... this data base, VISULEX searches for all the associated information. VISULEX offers two levels of detail. At the first level, the information is presented by using only the comments associated ... linguistic applications ; - as a debugging tool for linguistic applications ; - as a tool for converting the lexical base into a new form (for instance, loading it into a conventi...

Ngày tải lên: 17/03/2014, 19:21