Báo cáo hóa học: " A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses" pdf

... CATGATCAACTGCTCTGATTAC 1741(+) GGGCTTCCCGTACTTTGTG 2321(+) TGGATTTAGCTCCCTGAATG TYLCV primers for Q-PCR (176 bp amplicon) Ty 2164+ CTAAGAGCCTCTGACTTACTGC 200 nM Ty 2339- AACATTCAGGGAGCTAAATCCAG ... (A) in the multiple cloning site of the vector pGreen. (A) A T A A T T T A A T C (B) G C C G G/GATCCAAGCGGTCATCCGTATAATATTACCGGATGGCCGC/GGCCGCAAAAGAGCT/C C G BamHI NotI Sac...

Ngày tải lên: 20/06/2014, 01:20

... 95(5):2509-2514. 3. Hanahan D: Studies on transformation of Escherichia coli with plasmids. J Mol Biol 1983, 166(4):557-580. 4. Renaut L, Bernard C, D'Halluin JC: A rapid and easy method for production and ... Ad5-EGFP, Ad5-TK, Ad5-TK/EGFP and Ad5-E6mutFigure 1 Expression of EGFP, TK, TK/EGFP and E6mut in HeLa cells after transduction with Ad5-EGFP, Ad5-TK, Ad5- TK/EGFP...

Ngày tải lên: 20/06/2014, 01:20

... subjects, managed data acquisition and par- ticipated to drafting of the manuscript. AHK and MP con- ceived the study, evaluated the data, performed data analyses and wrote the manuscript. All authors ... investigated in a larger and more diverse sample of healthy and falls risk elderly adults. Surrogate data analysis The use of surrogate data was aimed at destroying the und...

Ngày tải lên: 19/06/2014, 10:20

... 5'-TAT- AGTCGACCACCCGGACTCAGAATCTCCT-3' and 5'- ATATGGATCCATCTCAGTCCCTCACAAGA-3' (HLA -A) , 5'-TATAGTCGACCACCCGGACTCAGAGTCTCCT-3' and 5'-ATATGGATCCATCTCAGTCCCTCACAAGA-3' ... TAP1), 5'- GTACAACACCCGCCATCAG-3' and 5'-GGACGTAGGG- TAAACGTCAGC-3' (TAP2), 5'-CTCGCGCTACTCTCTCTT- 3' and 5'-AAGACCAGTCCTTGCTGA-3' (β...

Ngày tải lên: 18/06/2014, 15:20

... study, experiments and data analysis, and multiple revisions of the manuscript. All authors read and approved the final version of the manuscript. Competing interests The authors declare that they have no ... access method offers a variety of advantages over traditionally synchronous access strategies and may be adapted to a wide variety of contexts, with primary relevance...

Ngày tải lên: 19/06/2014, 08:20

... conceived of the study, participated in the design, statistical analyses of the study and helped to draft the manuscript. All authors read and approved the final manuscript. Additional material Acknowledgements The ... material Acknowledgements The authors would like to thank Nisse Larson for excellent engineering assistance, Maria Frykman for valuable assistance during data coll...

Ngày tải lên: 19/06/2014, 08:20

... lack of a sensitive and multiplex approach for quantifying autoAb against a large panel of clinically relevant tumor-associated antigens (TAA). Methods: To circumvent preparation of phage lysates ... monoclonal Ab against human PSA (Biocon, Inc. Rockville, MD) to quantify total PSA levels. sero MAP-based PSA quantifi- cation was compared with standard ELISA-based PSA assays (Ame...

Ngày tải lên: 20/06/2014, 03:20

... maximum traffic demand of user i; r ij : transmission rate between user i and MR j; C max : maximum link capacity of a local access antenna; R j : local coverage of MR j; R max : maximum local ... 802.16 - Standard for local and metropolitan area networks, Part 16: Air Interface for Broadband Wireless Access Systems (2004) 35. H-Y Wei, S Ganguly, R Izmailov, ZJ Haas, Interfere...

Ngày tải lên: 20/06/2014, 22:20

... conjugate and h i is Gaussian random variable with zero mean and variance of N 0 /2. E b is the transmitted bit energy and ˆ a (iter) k is the estimated data of kth user at iter-th iteration stage. ˆ β i,m is ... VTC. 3, 2050–2054 (1997) 27. A Akansu, M Tazebay, M Medley, P Das, Wavelet and subband transforms: fundamentals and communication applications. IEEE Communications...

Ngày tải lên: 20/06/2014, 22:20

... Correspondence: balu7015@hotmail.com 1 Department of Physics, Wuhan University, Wuhan 430072, China Full list of author information is available at the end of the article Wang et al. Nanoscale Research Letters ... property for individual gas. The stability of sensor using Pd/ZnO nanorods was tested for air and insert gas of Ar, as shown in Figure 2c. The breakdown voltages of...

Ngày tải lên: 20/06/2014, 22:20