Báo cáo hóa học: " Detection and characterization of chicken anemia virus from commercial broiler breeder chickens" pdf
Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
... For EC and CY, reactions were initiated by addition of 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lgofGOU. For HP and M, reactions were initiated by addition of0 .1gofHPorMto1.0mLofVMcontaining10lgofGOU. ... amembrane fraction (M) from cultures of 2BW-1 (seeMaterials and methods). Enzymatic activity was determinedFig. 6. GC and GC-MS analysis of TMS-derivatives of metabolites produced from GOG by the ... levels of lignin are synthesized in wood and account for 15–36% of the dry weight of wood. Lignins are complex phenolicpolymers that reinforce the walls of certain cells in thevascular tissues of...
- 10
- 670
- 0
Báo cáo sinh học: "Detection and characterization of translational research in cancer and cardiovascular medicine"Ơ docx
... 9:57http://www.translational-medicine.com/content/9/1/57Page 2 of 12RESEARC H Open Access Detection and characterization of translationalresearch in cancer and cardiovascular medicineDavid S Jones1,2*, Alberto Cambrosio3 and Andrei Mogoutov1,4AbstractBackground: ... as the number of links between those two nodes (i.e., the number of timesX and Y were co-cited) divided by the square root of the product of the frequency of X and the frequency of Y in the overall ... analysis of pub-lication and citation data from tens of thousands of articlescan capture important features of active fields of scientificresearch, in this case in both cancer and cardiac medicine.The...
- 12
- 528
- 0
Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... and 215 nm, indicating that Rv1399c iscomposed of 22% o f a-helices, 25% of b-strand a nd 39% o frandom coil.The catalytic triad is located at the bottom of a smallpocket, 20 A˚length and ... 7.0).Ó FEBS 2004 Biochemical characterization of Rv1399c from M. tuberculosis (Eur. J. Biochem. 271) 3955Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosisA ... a8tostrandb8, a five-residues loop connecting strandb3 to helix a1 and two small h elices (a4anda6).The catalytic site consists of a functional catalytic triadfound in all serine enzymes of the...
- 9
- 584
- 0
Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... similarity of the A- and B-chains of the four isoforms, a pairwise alignmentwas performed and the values of identity expressed inFig. 3. (Continued). Characterization of four pulchellin isoforms ... Mrvalues of the standards in thousands. Lanes 2–9, 5 lg of peakP I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec-tively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol. ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig. 1C).Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 forthe four isoforms respectively.Secondary structure of the pulchellin isoformsand...
- 12
- 763
- 0
Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... Buchert1 and Kristiina Kruus11 VTT Technical Research Centre of Finland, Espoo, Finland2 Protein Chemistry Research Group and Core Facility, Institute of Biotechnology, University of Helsinki, ... Da). The identity of this peptide wasthen confirmed by MALDI-TOF ⁄ TOF fragment ionanalysis, where partial sequences of this peptide wereobtained from the ladders of b-fragment and y-frag-ment ... digestion of Coomassie Brilliant blue-stained protein bands from SDS ⁄PAGE was performed as described by Bamford et al. [22].MSMALDI-TOF MS was performed using an Ultraflex TOF ⁄TOF instrument...
- 14
- 650
- 0
Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D),wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O). Panel M is the control ... 7A, D, I and L are diamidino-2phenylindolehydrochloride (DAPI) stained cells showing the nuc-leus, and Fig. 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respect-ively.DiscussionNature ... (B and E), while Anti-PsCBL labelling is restricted tocytosol (J and M). (C and F) Superimposedimages of A, B, and D, E, respectively.Similarly, K and N are the superimposedimages of I, J and...
- 19
- 706
- 0
Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... 10.0;GAPEXT,0.05;GAPDIST,8;MAXDIV,40;ENDGAPS,off;NOPGAPS,off;NOHGAPS,off.Graphicaloutput of the bootstrap figure was produced by the programTREEVIEW.Isolation of RNA and RT-PCR analysisTotal RNA was isolated from RTG-2 cells and ... RT-PCR, were obtained from theNikko Branch of the National Research Institute of Aquaculture (Tochigi, Japan) and reared on a commercial diet at 15 °C.Cloning of HSFcDNAA random primed kZAPII ... for existence of twodistinct HSF1 isoforms in rainbow trout and the formation of heterotrimers of these isoforms in vitro.Materials and methodsCell culture and animalsRainbow trout gonadal fibroblast...
- 10
- 538
- 0
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... properties. The cause of these differences is not known.Km and kcatmeasurements of hevamine and mutantsComparison of the steady-state kinetic parameters of hevamine and the Tyr183Phe and Asp125Asn ... thecarbonyl oxygen atom of the N-acetyl group of the )1 sugarresidue has rotated away from the C1 atom of t hat residue.The c ombined s tructural and k inetic data sh ow thatAsp125 and Tyr183 contribute ... poly (N-acetylglucosamine), is one of the most abundant polymers in nature. It is a majorcomponent of the cell wall of yeast and other fungi, and theexoskeleton of arthropods. Although chitin is...
- 9
- 616
- 0
Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... a competitive mode of action.Uptake of Bip-[3H]Pro by SKPT cellsAfter characterization of Bip-Pro as a very high-affin-ity and enzymatically stable substrate of PEPT1 and PEPT2, the compound ... recovered from monolayers of both cell types intact to > 99% (HPLCdata not shown).Interaction of Bip-Pro with PEPT1 and PEPT2We next determined the interaction of Bip-Pro withPEPT1 and PEPT2 ... differ-ent Gly-Sar concentrations (50 and 500 lm in Caco-2cells and 10 and 100 lm in SKPT cells) in thepresence of increasing concentrations of Bip-Pro (0–100 lm and 0–50 lm, respectively). The...
- 10
- 490
- 0
Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... amide and carbonyl of Cys140 form hydro-gen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of theactive site cavity and the replacement of which ... of the vector by digestion with NcoI and PstI. This vectorbackbone was excised from an agarose gel and purified. Forboth standard and error-prone PCR amplification of hMnSOD genes, the following primers ... reassembly of DNase I-digested fragments, whereas an equal number of units of both Taq and Pfu DNA polymerases were used for theamplification of the reassembled product.Preparation of enzymesThe...
- 9
- 416
- 0
Từ khóa: báo cáo khoa họcbáo cáo môn học hóa dầubáo cáo trường học văn hóa năm 2012báo cáo trường học văn hóaquảng cáo trên báo giấy hoa học tròchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ