Báo cáo hóa học: " Molecular characterization of Umbre virus (Bunyaviridae)" pdf

... (A) Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identified in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position ... 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the function of a diverse number of RNA-binding proteins...

Ngày tải lên: 18/02/2014, 06:20

... Munn 2,3 and Hongyuan Yang 1 1 Department of Biochemistry, Faculty of Medicine, National University of Singapore, Republic of Singapore 2 Institute of Molecular and Cell Biology, A*STAR Biomedical ... its mature form (M form with molecular mass of 61 kDa). Deficiency in this process leads to accumulation of and secretion of p2 CPY. As shown in Fig. 7, at 0 min of chase, p...

Ngày tải lên: 07/03/2014, 21:20

... between 71% and 95% of the amount Fig. 3. SDS/PAGE of extracts of recombinant E. coli containing NoxA-1, NoxB-1 or NoxC from A. fulgidus. M, Calibration proteins. The molecular mass of the calibration ... 2887 Molecular characterization of H 2 O 2 -forming NADH oxidases from Archaeoglobus fulgidus Serve ´ W. M. Kengen, John van der Oost and Willem M. de Vos Laboratory of Mic...

Ngày tải lên: 08/03/2014, 02:21

... of a c-carbonyl of histone H2B by esterification, followed by a nucleophilic attack by an e-amino group of Lys residue of histone H4 [38], the fact that the cross-link is formed between Gln9 of ... that of the catalytic site-containing region of the other TGs was amplified. To obtain further sequences upstream of primer TG5 and downstream of primer TG3V, we used the r apid amp...

Ngày tải lên: 08/03/2014, 10:20

... of ticks, for which the blood-meal from the host is the only source of energy. Elucidation of the function of HlSCP1 in the hard tick may contribute a better understanding of the physiology of ... a molecular mass of 53 kDa and a pI of 7.5 (Fig. 2A), corresponding to the predicted size of the putative pro- tein calculated from the HlSCP1 amino acid sequence. To detect the...

Ngày tải lên: 16/03/2014, 10:20

... using IMAGEQUANT software (Amersham Biosciences). Standards consisted of 5 lL aliquots of serial dilutions of the reaction mixtures, with the moles of phosphate defined using the ATP c oncentration. Division of ... low levels of phosphorylation effect- ively preclude further biochemical characterization, such as the identification of phosphorylation sites or the assessment of a...

Ngày tải lên: 16/03/2014, 18:20

... experiments. On the basis of Table 1. A comparative analysis of thermodynamic parameters of ESAT–CFP-10, Rv0287–Rv0288, and Rv3619c–Rv3620c. The stoichiome- try of interaction and values of K b and DH were ... measurements of the interaction between Rv3619c and Rv3620c in phosphate buffer at 25 °C; raw data of heat effect (lcalÆs )1 ) of 30 injections (10 lL each) of 0.1 mM...

Ngày tải lên: 22/03/2014, 16:21

... ª 2008 The Authors Journal compilation ª 2008 FEBS Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by ... ratio of Vg to actin transcripts in the GIH-depleted background suggested that functional knockdown of Pem-GIH led to the induced expression of Vg in the ovary. Discussion Because...

Ngày tải lên: 23/03/2014, 07:20

... amino-acid sequence of the artemin monomer consists of 230 residues with a calculated molecular mass of 25 976 Da. The ferritin cDNA (accession number AY062897) of 725 bp consists of an ORF of 516 bp, ... MacRae 1 1 Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada; 2 Department of Molecular Cell Biology, Sylvius Laboratory, Leiden, the Netherlands;...

Ngày tải lên: 23/03/2014, 20:22

... expected, a protein of molecular weight 49 kDa was induced from transformant bearing the p19C-S in the presence, but not in the absence, of IPTG. For immunization, a protein of molecular mass 49 ... the presence of a putative Mig1p binding site in the promoter of MRG19 (http:// cgsigma.cshl.org/jian/). However, our results do not exclude the possibility of glucose inactivation...

Ngày tải lên: 31/03/2014, 08:20