Báo cáo hóa học: " Secretion of Epstein-Barr Virus-encoded BARF1 oncoprotein from latently infected B cells" doc

... BioMed Central Page 1 of < /b> 4 (page number not for citation purposes) Virology Journal Open Access Short report Secretion < /b> of < /b> Epstein-Barr < /b> Virus-encoded < /b> BARF1 < /b> oncoprotein < /b> from < /b> latently < /b> infected < /b> ... purified by concanavalin A affinity column. BARF1 < /b> was transcribed in both EBV-positive AKATA and IB4 cells, and BARF1 < /...

Ngày tải lên: 20/06/2014, 01:20

... shows nor- thern blots of < /b> the selected clones, using 32 P-labelled cDNA probes of < /b> serglycin, MMP-9, uPA and 3 6B4 , cultured in both absence and presence of < /b> PMA. The northern blot of < /b> the unstimulated ... fluoride) (PVDF) membranes. After blocking with 5% skim milk, the membranes were incubated with a rabbit polyclonal antibody to human MMP-9 (Ab-10, NeoMarkers, Frem...

Ngày tải lên: 30/03/2014, 11:20

... can not be adequately addressed in a short-term test, we performed outgrowth assays that evaluate the ability of < /b> a fixed input of < /b> T cells to inhibit long-term growth of < /b> different numbers of < /b> target ... complete medium. Generation of < /b> EBV-specific CD4 + and CD8 + T-cell lines EBV-specific T cells were established as previously described [ 10], with modification...

Ngày tải lên: 18/06/2014, 16:20

... Tables Table 1 - Details of < /b> subjects Subject Group Age Gender Disease/injury duration Clinical classification Walking speed (m/s) 1 AB 25 F – – 0.714 2 AB 22 F – – 0.667 3 AB 24 ... particular signatures of < /b> pathological gait. It is often desirable to compare one set of < /b> SEMG waveforms to another in order to determine if a subject’s gait exhibits abnormal b...

Ngày tải lên: 19/06/2014, 08:20

... day p.o. and then increased by 15 mg biweekly to 45 mg per day p.o. for the duration of < /b> the treatment. Safety was assessed by measurements of < /b> metabolic profiles, blood pressure, and edema; effects ... Neurology, University of < /b> Bonn, Bonn, Germany, 3 Department of < /b> Internal Medicine, Kern Medical Center, Bakersfield, California, USA and 4 Department of < /b> Anesth...

Ngày tải lên: 19/06/2014, 22:20

... difficult. Other areas of < /b> concern in most of < /b> the studies included the use of < /b> FEF, a less stable measure of < /b> obstruc- tion[12,22,33,36], incomparable or absent control groups, [18,23,32], lack of < /b> DLCO measurement ... unsolved. Begin et al reported peribrochial alveoli- tis, in high dose crysotile asbestos exposed sheep and fibrosis with obliteration and narrow...

Ngày tải lên: 20/06/2014, 00:20

... Tris-buff- ered saline and blocking with blocking solution (2% bovine serum albumin and 0.05% Tween 20 in TBS), the immobilized viruses were incubated with anti-penta-His 6 tag monoclonal antibody ... Jullienne B, Esselin S, Connault E, Opolon P, Tordjmann T, Vigne E, Perricaudet M, Benihoud K: Substitution of < /b> hexon hypervariable region 5 of < /b> adenovirus serotype 5 abro- gates bl...

Ngày tải lên: 20/06/2014, 01:20

... post-electroporation. Cells were blocked in 5% mouse serum prior to incubation with rabbit polyclonal anti-SFV Ab, Ab-6KTF-N or Ab-TF-C overnight at 4°C. After washing, cells were then incubated with biotinylated mouse ... mounting medium (Vector Labs) and analyzed by fluorescence microscopy. List of < /b> abbreviations AURAV: Aura virus; BEBV: Bebaru virus; BFV: Barmah For- est virus; CHIKV...

Ngày tải lên: 20/06/2014, 01:20

... infected < /b> cell proteins; Lane 4: aMPV /B infected < /b> cell proteins; Lane 5: aMPV/C infected < /b> cell proteins. B. Antibody detection of < /b> a C-terminal portion of < /b> the aMPV/C nucleoprotein. Lane 1: uninfected ... saline (PBS). Affinity purified rabbit anti-peptide antibody (diluted 1:100) was used as the source of < /b> the pri- mary antibodies and incubated for 1...

Ngày tải lên: 20/06/2014, 04:20