Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot

... virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug (N)-methanocarbathymidine Mark N Prichard* 1 , Earl ... contributed to the design of the experiments and analysis of the data. KAK contributed to the acquisition and interpretation of data. RWM co...

Ngày tải lên: 20/06/2014, 01:20

... marker in the presence of Geneticin G418 sulfate (Invitrogen). The second recombination of NEO R -con- taining viruses occurred in the absence of Geneticin G418 sulfate, producing a wild type virus ... bovine serum (BD Bio- sciences), 2 mM Glutamax (Invitrogen), and 1% penicil- lin-streptomycin (Invitrogen), and incubated as described above. Blasticidin (5 µg/ml, Invitrogen) w...

Ngày tải lên: 20/06/2014, 01:20

... triggers G to A hypermutations in the newly synthesized viral DNA. The inhibition of viral replication is due either to degradation of the cDNA by the DNA repair machinery or to the lethality of the ... APOBEC3G-induced mutational pressure [9]. We were, therefore, interested to determine whether APOBEC3G is also a restricting factor for this virus. Results and Di...

Ngày tải lên: 20/06/2014, 02:20

... immune responses in humans. Further improvements of the recombinants, using the E6 and E7 transgenes deleted of the p53 and p105Rb cellular binding domain, might fur- ther increase the safety of the vaccine. ... the best choice of interven- tion against HPV, as they can inhibit infection and pre- vent clinical disease by neutralising the incoming virus. On this basis,...

Ngày tải lên: 18/06/2014, 16:20

... Jahrling PB: Apoptosis induced in vitro and in vivo during infection by Ebola and Marburg viruses. Lab Invest 2000, 80(2):171-186. 41. Lewis J, Wesselingh SL, Griffin DE, Hardwick JM: Alphavirus- induced ... both in vitro and in vivo [38,42-44]. These observations suggest that virus- induced apoptosis may contribute to the patho- genesis of Dengue. While the molecular...

Ngày tải lên: 20/06/2014, 01:20

... (CBNV) in the Chinese mole shrew [6], Tan- ganya virus (TGNV) in the Therese shrew (Crocidura there- sae) [7], and Ash River virus and Jemez Springs virus in the masked shrew (Sorex cinereus) and the ... less similar to TPMV VRC-66412 (49.3% at the nucleotide and 44.2% at the amino acid level). As in other soricid-borne hantaviruses discovered to date, t...

Ngày tải lên: 20/06/2014, 01:20

... (p17K) into a 17kDa cleavage product (17K) [4]. Its proteolysis is similar to the processing of the other VV core proteins in that the cleav- age is sensitive to rifampicin, takes place at the conserved recognition ... expression with the AG/A site mutation (AG/A) into ID/I, however, failed to complement the absence of A12L protein, resulting in the similar virus...

Ngày tải lên: 18/06/2014, 18:20

... randomly selected and then invited to participate in the trial. There are two types of PRO that can be used to measure HRQOL: generic and disease-specific. Generic instru- ments are designed to ... improved approach to GERD management in the primary care set- ting, tailoring treatment on an individual basis in order to lessen the impact of the disease. This may be...

Ngày tải lên: 18/06/2014, 18:20

... developing and evaluating the OIDP and the OSS for the Spanish population consisted of three main steps: linguistic and cultural adaptation of the orig- inal OIDP to the Spanish setting using the ... fac- tors. This study is the first to use the OIDP index in a Spanish population and the first OHRQoL study in Spanish adults. Both instruments (OIDP and OSS...

Ngày tải lên: 18/06/2014, 19:20

... VP2. The capsid is divided into two domains the N- terminal shell (S) and the C-terminal protusion (P), linked by an eight amino acid hinge. The P domain con- sists of the P1 and P2 subdomains. The ... using this virus against other noroviruses in the database [GenBank] in order to identify differences, if any, between Carlow virus and other viruses circulating...

Ngày tải lên: 20/06/2014, 01:20