Báo cáo hóa học: " Universal primers that amplify RNA from all three flavivirus subgroups" pdf

... Access Research Universal primers that amplify RNA from all three flavivirus subgroups Sheryl L Maher-Sturgess 1 , Naomi L Forrester 2 , Paul J Wayper 3 , Ernest A Gould 2 , Roy A Hall 4 , Ross T Barnard 4 ... flaviviruses. Previous attempts to analyse the entire genus using PCR, have required multi- ple sets of primers. The capacity of the Flav100R and Flav200R primers...

Ngày tải lên: 20/06/2014, 01:20

... yielding low BxB frequency in selected primers. All these primers gave negative result when they were tested in UCSC in silico PCR to see whether primers would amplify human DNA. Table 1: Candidate ... set of primers were proved to have a good PCR efficiency. The four sets of fragment primers are also based on the most conserved regions from public sequences. These primers ar...

Ngày tải lên: 20/06/2014, 01:20

... yielding low BxB frequency in selected primers. All these primers gave negative result when they were tested in UCSC in silico PCR to see whether primers would amplify human DNA. Table 1: Candidate ... set of primers were proved to have a good PCR efficiency. The four sets of fragment primers are also based on the most conserved regions from public sequences. These primers ar...

Ngày tải lên: 18/06/2014, 18:20

... ranged from 0.69 – 0.82 for individual plates, and the aggregate Z' value from all plates and all days was 0.64. A signal-to-noise value of 4.96 was calculated with the aggregate data from all ... provided template [14]. Three individual experiments, each containing three plates, were conducted on three separate days. A) The left panels show a representative set of data...

Ngày tải lên: 20/06/2014, 01:20

... al.: Universal screening of Tanzanian HIV- infected adult inpatients with the serum cryptococcal antigen to improve diagnosis and reduce mortality: an operational study. Journal of the International ... 0.05 to be statistically significant. Ethical issues Ethical approval was obtained from the BMC and Weill Cornell Medical College IRBs and a written informed consent was obtained from ea...

Ngày tải lên: 20/06/2014, 08:20

... prove that the technique is universal in the sense that the required number of measurements for accurate recovery is optimal and indep endent of the sparsity basis. This universality stems from ... chirp rate ¯w that ensures that the product N w µ 2 w is at its minimum. This minimum might change dep ending on the sparsity matrix, so the universality of the recovery is formally los...

Ngày tải lên: 21/06/2014, 17:20

... are called mostly interacting residues (MIRs). Their selection requires fixing a cut-off value above the mean value NC. It was found that NC(i) varies b etween 1 a nd 8 a nd that NC ¼ 4 for all ... f all 111 proteins. The most probab le value is four, w hich coincides with the mean sequence value, which is also fou r for all proteins as stated above. From this distribution, it appea...

Ngày tải lên: 07/03/2014, 16:20

... of ECs and MCs derived from human ES cells; hES-ECs: ECs derived from human ES cells; hES- MCs: MCs derived from human ES cells. Competing interests The authors declare that they have no competing ... analysis of mRNA expression of VE-cadherin in hES-MCs, hES- ECs and HUVECs. Total cellular RNA was isolated from hES-MCs, hES- ECs and Human umbilical vein endothelial cells (HUVECs)...

Ngày tải lên: 18/06/2014, 15:20

... IL-2 [30], suggesting that their effects may be mutually exclusive as opposed to redundant. There is evi- dence that CTLA4 is expressed on murine Th17 cells at lev- els that are higher than Th1 ... ranges of detection were from 6.9 to 5000 pg/ml for IL-4, IL-5, IL-6, IL-10, IL-13, TNF-α, from 12.3 to 9000 pg/ml for INF-γ and MCP-1, from 4.1 to 3000 pg/ml for RANTES and from 3.9...

Ngày tải lên: 18/06/2014, 15:20

... successfully obtained from EGDSs and from each biopsy a similar amount of tissue weight was processed from CD and non-CD (54.9 mg ± 6.4 vs 56.7 mg ± 4.8; respectively; p = ns). All the CD patients ... culture, FB-like cells growing radially from the chopped and enzymatically digested bioptic pieces were observed; their lateral spreading increased dimensionally during the culture and...

Ngày tải lên: 18/06/2014, 15:20