Báo cáo hóa học: " Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method" pptx

... purposes) length of 5'UTR was same as that of other type 4 isolates and had nucleotide G at the extreme 5' end of the genome as other reported genotype 4 sequences. Whole genome- based ... constructed using MEGA software depicting genotypic status of SH-SW-zs1 on the basis of full- length genome sequence of 39 HEV isolatesFigure 2 Phylogenetic trees constructe...

Ngày tải lên: 20/06/2014, 01:20

... sequence of 39 HEV isolatesFigure 2 Phylogenetic trees constructed using MEGA software depicting genotypic status of SH-SW-zs1 on the basis of full- length genome sequence of 39 HEV isolates. Genbank ... purposes) length of 5'UTR was same as that of other type 4 isolates and had nucleotide G at the extreme 5' end of the genome as other reported genotype 4 se...

Ngày tải lên: 18/06/2014, 18:20

... the MDCK sequence. Yet, when the sequences encompassing this region were aligned to the sequences adjacent to known RNA pol I transcription initiation site sequences of other species, conserved ... (green). The sequences from +2 to +9 (blue) are conserved in the indicated mamma- lian species. Based on the aligned sequences, the G residue (arrow) in the MDCK sequences was predicted to be...

Ngày tải lên: 20/06/2014, 01:20

... acid sequences from 9 genes sequences were aligned by Mega 4. Amino acids that are identical between CSN and other sequence are shaded in black and the two conserve regions are underlined. The ... described above. Sequence analysis and phylogenetic tree Obtained csn sequence was analyzed for the identity and similarity with other related sequences by BLAST on-line. The signal...

Ngày tải lên: 21/06/2014, 19:20

... two selected cell clones was markedly decreased, which demonstrated that RNAi technique was an effective way to modulate the ABCC2 expression in CNE2 cell line. The selected cell clones, CNE2/shABCC2-1 ... as described above. Each sample was measured at least three times. Analysis of ABCC2 protein levels by western blot After proteins of all cells were prepared, western blot method was pe...

Ngày tải lên: 18/06/2014, 15:20

... cells were used as negative control target cells. Peptide-specific interferon-γ release of CTLsFigure 7 Peptide-specific interferon-γ release of CTLs. PBMCs of HLA-A24-positive prostate cancer ... time. Interestingly, the immunogenic peptides were distinct among the patients. However, it is likely that the AMACR2 peptide was the most immunogenic of the three AMACR-derived peptides. T...

Ngày tải lên: 18/06/2014, 15:20

... may be achieved with patient benefit [15]. Lastly, if fully tested under Strategy 3, where suicide genes are generally unneeded, a 2 nd generation designer T cell does not require a suicide gene ... exposure of G250 T cells been by Strategy 4, the consequences could have been dire. Strategy Escalation With these options, it can be seen that there are now choices, not just of dose level...

Ngày tải lên: 18/06/2014, 16:20

... susceptible alleles [22]. The protection conferred by the Idd3 C57BL/6 allele can be explained by the pre- sence of 46 SNPs upstream of the minimal promoter of the IL-2 gene that can alter the transcriptional ... treatment of several diseases where the immune system necessitates strengthening of the activated T cell pool. As such, IL-2 is a frequent therapy in the treat- ment of s...

Ngày tải lên: 18/06/2014, 16:20

... is difference between these two observers, these slides were reinvestigated by both investigators using a multi- headed microscope. Tumors with more than 5% of POLh-positive cancer cells were regarded ... poor treatment response and shorter survi- val in patients of above settings. DNA polymeraseh iscodedbyPOLHgenewhichis one of the 150 human DNA repair genes, whose defec- tion result...

Ngày tải lên: 18/06/2014, 16:20

... model lays Naïve phenotype Activated, central memory / reduced effector phenotype Activated, peripheral memory / enhanced effector phenotype Excessively activated, anergic / exhausted p henot yp e Epitope-specific ... that encompasses simple vectors with well-defined MHC class I-restricted epitopes. Using a Melan A/MART-1 preclinical experimental model, we developed a str ategy that g...

Ngày tải lên: 18/06/2014, 16:20