Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx

... between bovine cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain. In bovine cell lines, serial viral passages analysis and infection with both T1 and SG33 expressing ... JL, Esteban M: Differences in virus- induced cell mor- phology and in virus maturation between MVA and other strains (WR, Ankara, and NYCBH) of vaccinia vir...

Ngày tải lên: 20/06/2014, 01:20

... between bovine cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain. In bovine cell lines, serial viral passages analysis and infection with both T1 and SG33 expressing ... of 5 (page number not for citation purposes) Virology Journal Open Access Short report In vitro permissivity of bovine cells for wild-type and vaccin...

Ngày tải lên: 18/06/2014, 18:20

... fusion cells producing TGF-β resulted in the induction of Treg in vivo and in vitro in a murine model [52]. Moreover, the blockade of TGF-β reduces Treg induction by the fusion cell vaccine and ... cytometry for expression of WT1 and HLA-DR. Numbers represent cells positively staining for the indicated surface markers. C, Percent- age of cells positive for...

Ngày tải lên: 18/06/2014, 15:20

... transferrin was adequate for growth of Vero and SK-N-SH cells for 10–12 days. Se-deficient cells were maintained for 10 days and pas- saged every 3 days using serum-free trypsin-EDTA solu- tion and ... concentration of LDH was expressed as fold-change in LDH activity in WNV-infected cells with respect to their respective mock-infected cells. Apoptosis in WNV-infec...

Ngày tải lên: 20/06/2014, 01:20

... to determine the role, if any, of chlo- roquine in the prophylaxis and treatment against influenza infection. It would also be interesting to deter- mine the activity of chloroquine against the ... applied to inhibit viral replication. We report here that chloroquine is able to inhibit influenza A virus replication, in vitro, and the IC50s of chloroquine against influenza A v...

Ngày tải lên: 20/06/2014, 01:20

... cells) , fixed in buffered formalin and embedded in paraffin. a, b: Staining with H&E. c, d: Specific brown staining of apoptotic cells by anti-caspase 3 Ab. e, f: In situ end-labeling (ISEL); ... grown in vitro under three-dimensional condi- tions using a mixture of collagen and fibroblasts [32,44]. Interaction of a2andb1 integrins on CRC-specific T cells with co...

Ngày tải lên: 20/06/2014, 03:20

... 5 min of incubation with 0.4% Trypan blue, the percentages of stained cells (indicative of nonviable cells) versus stain-excluding cells were counted in a hemocytometer. Then the percentage of ... 70°C for 3 min, and placed on ice until the addition of the remaining reaction components. The reaction was incubated at 42°C for 90 min, and terminated by heat inactivation...

Ngày tải lên: 20/06/2014, 04:20

... and its binding affinity is not altered by the presence of DNA in the DNA binding site. Interestingly, when 3 n M RNA and 10 n M DNA were added simultaneously to a limiting amount of protein in TK8 ... rate constant for the binding of DNA to protein kinetically and obtained a value of at least half this in TK8 buffer containing 10 m M MgCl 2 [33]. Therefore, given that th...

Ngày tải lên: 17/03/2014, 10:20

... with 2 × 10 6 cells in 0.2 mL of media containing 20% Matrigel ™ (Becton Dickinson, Bedford, MA, USA) or intraperitoneal (i.p.) injections of 1×10 8 cells in 1 mL of media. Animals bearing s.c. tumors ... MW: A new and convenient method for purification of Y-86 using a Sr(II) selective resin and comparison of biodistribution of Y-86 and In- 111 labeled Herceptin (...

Ngày tải lên: 21/06/2014, 02:20

... Parasites were cultured in medium M199 with 10% foetal bovine serum; 25 mM of HEPES, and 0.22 μg of penicillin and streptomycin, respectively at 24°C in an incubator. 1 mg of each test compound ... indicating deprotonation of ligand. In the spectra of compounds V-VIII, appearance of ne w band of med- ium intensity around 430 cm -1 indicated the coordina- tion from N...

Ngày tải lên: 21/06/2014, 02:20