Báo cáo hóa học: " Attenuation and efficacy of human parainfluenza virus type 1 (HPIV1) vaccine candidates containing stabilized mutations in the P/C and L genes" docx

... cells, making manufacture of these vaccines commercially feasible. In the present study, two new rHPIV1 viruses containing single att mutations in L, L 17 10 11 and L Y942A , were generated and ... to the level of temperature sensitivity in vitro and attenuation in vivo. The rHPIV1 mutant bearing the indi- vidual att mutation L 17 10 11 (rHPIV1-C R84G...

Ngày tải lên: 20/06/2014, 01:20

... HPIV1 wt and rHPIV1 mutant viruses containing the indicated mutations in the P/C, HN and L genes in a multiple cycle growth curve. Monolayer cultures of LLC-MK2 cells and Vero cells were infected ... cells, making manufacture of these vaccines commercially feasible. In the present study, two new rHPIV1 viruses containing single att mutations in L, L 17 1...

Ngày tải lên: 18/06/2014, 18:20

... the isolates with amino acid profile of 75-I, 97 -L, 13 9-Q and 14 4-Q were clustered together in cluster I while the remaining five isolates with amino acid profile of 75-V, 97-M, 13 9-K and 14 4-E ... current isolates including previously char- acterized Malaysian isolates BL-5 [12 ] and 3 -1 [13 ] had amino acid profile of 75-V, 97-M, 13 9-K and 14 4-E. These iso...

Ngày tải lên: 20/06/2014, 01:20

... well as the mechanism of the underlying intracellu- lar translocation. Localization of different types of virus particles during virus maturation is currently made by hand. Structural aspects of ... could also be substituted for truncation, thereby allowing weighting of the norms and inner prod- ucts. Conclusion Monitoring the in- cell structural morphology of viru...

Ngày tải lên: 20/06/2014, 01:20

... 11 2Female3AASevere 11 3 Female 5 C Severe 6 ,11 4 Female 6 C Severe 6 ,11 5Female3AASevere 11 6Male5AASevere6 ,11 7 Female 12 AA Mild 11 8 Female 10 AA Severe 6 ,11 9Male8AASevere 11 Adult 10 Male 57 C Severe 6 ,11 11 ... Chang TL, Vargas J Jr, DelPortillo A, Klotman ME: Dual role of alpha-defensin -1 in anti-HIV -1 innate immunity. Journal of Clin- ical Investigation...

Ngày tải lên: 20/06/2014, 01:20

... Identification of the xenobiotic-metabolizing enzyme arylamine N-acetyltransferase 1 (NAT1) as a new target of cisplatin in breast cancer cells: molecular and cellular mechanisms of inhibition. Mol Pharmacol 73, ... used clinically in the treatment of chronic alcoholism since 19 48 [1] (Fig. 1) . DS acts by irreversibly inhibiting the hepatic aldehyde dehydro- genase, l...

Ngày tải lên: 07/03/2014, 02:20

... dense particles in the following. The use of polyclonal antibodies recognizing hIRP1 (Fig. 2B,C) resulted in heavy and uniform labelling of all inclusion bodies, which clearly indicated the associ- ation ... stained. Lane 1, recombinant human IRP1; lanes 2– 11 , fractions 1 10 of the sucrose gradient. (B) Western blot of the same fractions. Lane 1, recombinant h...

Ngày tải lên: 07/03/2014, 10:20

... including glucosamine and chondroitin, one month prior to study and during the study, other than multivitamin and mineral supplements containing vitamins and minerals as the sole medicinal ingredients ... typically worsens with weight bearing, including walking and standing, and improves with rest (4). Other symptoms include morning stiffness and gelling of the invo...

Ngày tải lên: 26/10/2012, 09:48

... dishes in RPMI con- taining 10 % FBS, 2 uM L- glutamine and 50 IU/ml penicil- lin and 50 ug/ml streptomycin at a density of 10 million cells/plate. After allowing cells to settle for 90 minutes, the cells ... with LXR-623. In both rats and humans given a single dose of LXR-623, the induction of ABCA1 and ABCG1 expression in peripheral blood cells tracked closely wit...

Ngày tải lên: 18/06/2014, 15:20

... onto OP9-Del1 cells seeded one day before, in 24-well plates at 3 × 10 4 cells/well. Cells were cultured in RPMI 16 40 (Inv- itrogen) supplemented with 10 % FCS (Hyclone), 1% L- glutamine and 1% Penicillin/Streptomycin ... Liu YJ: The nature of the principal type 1 inter- feron-producing cells in human blood. Science 19 99, 284 :18 35 -18 37. 16 . Liu YJ: IPC: p...

Ngày tải lên: 18/06/2014, 15:20