... μg/Lpenicillin in a humidified incubator containing 5% CO2at 37°C. The immortalized esophageal cell line NE-3was obtained from Dr. Jin (the University of HongKong, P. R. China)[26] and cultured in ... mitotic kinase CDK1/cyclin B complex in the cytoplasm to stimulate cell cycle progression [16].Furthermore, overexpression of CDC25B has beenobserved in a variety of human cancers, including ... of Type Culture Collection of Chinese Academy ofSciences, Shanghai, China) were grown in RPMI 1 640(Invitrogen, Carlsbad , CA) supplemented with 10% fetalbovine serum, 100 μg/L streptomycin,...