... rVP1. Interestingly, that study found a single amino substitution was critical for the for-mation of VLPs, whereas our results showed that a singleamino acid substitution was beneficial, i.e., increased ... transcription, leading to increased yields of SaVVLPs in insect cells. Furthermore, many of these studiesthat expressed calicivirus rVP1 in insect cells only exam-ined rVP1 expression and yields of VLPs ... dpi onto a 2% denaturing agarose gel con-taining formaldehyde. The amounts of total RNA werecompared using SYBR Gold staining (Invitrogen, USA).RNA was transferred to a positively charged nylon...