... (JE) virus is the most com-mon among them in the world [1]. In India, JE, the lead-ing cause of viral encephalitis, has been endemic in SouthIndia, since 1978. The mortality of this infection ... were adapted to porcine kidneycell line in the laboratory by serial blind passage. Porcinekidney cell line infected with JE, WN and Dengue-2viruses stained by indirect immunofluorescence served ... were included in every run of the assay for comparison. Smears were examined by two independent examiners and recorded.Standardization of Shell vial cultureAfter adaptation of JE, WN and Dengue-2...