báo cáo hóa học: " Neuropeptide Y inhibits interleukin-1b-induced phagocytosis by microglial cells" ppt

... 8:169 http://www.jneuroinflammation.com/content/8/1/169 Page 8 of 15 Figure 1 NPY inhibits bead phagocytosis by microglial cells. (A) Represe ntative photomicrogra phs illustrate the inhibi tory effect of NPY on LPS-induced phagocytosis. (B) LPS (100 ... experimental model of microglial phagocytic activity induced by lipopolysac- charide (LPS) to evaluate the role of NPY on Fc re...

Ngày tải lên: 19/06/2014, 22:20

... with 10 μl antibody to RNA Polymerase II (sc-900; Santa Cruz Biotechnology); 10 μl antibody to RPA 70 (sc-25376; Santa Cruz Biotech- nology) or 7.5 μl antibody to hyperacetylated H4 (06–866 Upstate) ... inhibitors of HDAC activity. NaBu, a short chain fatty acid occurring naturally in the body, is a byproduct of anaerobic bacterial fermentation of dietary fiber [5,6]. TSA, a hydroxamic acid i...

Ngày tải lên: 20/06/2014, 01:20

... physical states determined by the READ voltage. These two different physical states can be inter- preted as Boolean logic states. Therefore, the system can be used as a random access memory (RAM) ... [18] always contain more than millions of atoms. Consequently, MD has difficulties in studying the feasibility of the proposed NEMS design in practical applications. In this paper, we employ a c...

Ngày tải lên: 22/06/2014, 01:20

... activity. NPY is able to act through five different receptors (Y1 , Y2 , Y4 , Y5 and y6 ) that vary in their binding profiles and in their distribution in the central nervous system and periphery. Y1 , Y2 and ... bone activity by this neuropeptide [66]. However, one should bear in mind that, although NPY is their main ligand, the Y receptors can also be activated by peptide YY an...

Ngày tải lên: 18/02/2014, 04:20

... ovary; cNPY, chicken neuropeptide Y; cPP, chicken pancreatic polypeptide; cPYY, chicken peptide YY; Hsa, Homo sapiens chromosome; pNPY, porcine neuropeptide Y; PP, pancreatic polypeptide; pPYY, ... > cPP (see Table 1). Interestingly, pNPY had a lower affinity than pPYY, thereby making it unlikely that cNPY would bind with higher affinity (they differ by only two conservative replacem...

Ngày tải lên: 19/02/2014, 07:20

... Gorrell 1 1 Centenary Institute, Sydney Medical School, University of Sydney, NSW, Australia 2 Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sydney, NSW, Australia Keywords antiplasmin-cleaving ... neurobiology. Structured digital abstract l FAP cleaves GLP-1-amide by protease assay (View interaction) l DPP4 cleaves PACAP by protease assay (View interaction) l FAP cleaves P...

Ngày tải lên: 14/03/2014, 23:20

... periosteum and bone marrow by RIA [13], particularly in megakaryo- cytes [14]. Recently, it was additionally reported that the NPY receptor Y1 , but not Y2 , Y4 , Y5 or Y6 , was expressed in cultured ... embryonic stages and in the adult, that NPY is synthesized by osteoblasts, osteocytes, and chondrocytes. Moreover, peptidylglycine a-am- idating monooxygenase, the enzyme responsible...

Ngày tải lên: 15/03/2014, 09:20

... [3]. In the present study we examined the neurotox- icity of fibrillar peptides by exposing the SH-SY 5Y neuroblastoma cell line, or murine primary cortical neu- rons, to amyloid-β 1-42 , to a peptide ... M, Ferrari D, Rossi F: Activation of microglial cells by beta-amy- loid protein and interferon-gamma. Nature 1995, 374:647-650. 18. Oyama Y, Fuchs PA, Katayama N, Noda K: Myricetin a...

Ngày tải lên: 19/06/2014, 22:20

... fluorescence. Flow cytometric analysis The pDC phenotype was assessed using three color immu- nostaining with biotinylated, phycoerythrin (PE)-, Cy- Chrome (CyC)-and allophycocyanin (APC) -conjugated monoclonal ... medium and analysed by flow cytometry between day 5 and day 60. Transduction of CD34-pDC Semi-adherent and non-adherent cells in culture were har- vested at day 6 and transduced...

Ngày tải lên: 18/06/2014, 15:20

... specificity of the PCR products was confirmed by melting curve analysis, by gel electrophoresis using the AlphaEaseFC Imaging software (Alpha Innotech, San Leandro, CA) and by sequencing. Data analysis/statistical ... esti- mated by the 2-sided Mann-Whitney U test for compari- son of two different groups. Detection of PAX2 proteins by Western Blot Western blots were performed on equ...

Ngày tải lên: 18/06/2014, 15:20